DNA methylationThe large amount of DNA in eukaryotic chromosomes is duplicated exactly for distribution to daughter cells at division. Unlike prokaryotes, the eukaryote must co-ordinate multiple initiations of replication on a single molecule so as to replicate the DNA once, and only once, in a ...
Despite the fact that rapid advances have been made in deciphering the detailed molecular mechanism of DNA replication in prokaryotes (1), parallel progress has not been made in eukaryotic systems. This has been due in great part to the inherent complexity of eukaryotic cells, the lack of well...
The process of DNA replication contains many steps. The first step is the necessity for the double-stranded DNA molecule to be opened up into single strands by the enzyme DNA helicase, which makes a replication fork. The second step is the addition of ...
Fewer protein factors (designated IF) participate in prokaryotes. In eukaryotes, several steps occur in succession: Step 1. Initiator Met-tRNA and the GTPase eIF-2A (with bound GTP) form a preinitiation complex on a small ribosomal subunit. Step 2. Several protein initiation factors assemble...
Divided genomes: negotiating the cell cycle in prokaryotes with multiple chromosomes. Historically, the prokaryotic genome was assumed to consist of a single circular replicon. However, as more microbial genome sequencing projects are comple... ES Egan,MA Fogel,MK Waldor - 《Molecular Microbiology》 ...
Role of RNA polymerase and transcription in the organization of the bacterial nucleoid. One of the major structural differences that provides a taxonomic distinction between eukaryotic and prokaryotic organisms is that prokaryotes lack the nuc... DJ Jin,C Cagliero,YN Zhou - 《Chemical Reviews》 被引...
In DNA replication, where does replication begin and end? A) Origin of replication; end of the molecule B) Promoter; termination site C) Start codon; stop codon D) Start codon, termination siteWhat is the nucleotide sequence of an mRNA str...
Genes and Development: a Journal Devoted to the Molecular Analysis of Gene Expression in Eukaryotes, Prokaryotes, and VirusesRiera A, Barbon M, Noguchi Y, Reuter LM, Schneider S, Speck C. 2017. From structure to mechanism- understanding initiation of DNA replication. Genes & Development 31:1073...
The initiation of chromosomal replication occurs only once during the cell cycle in both prokaryotes and eukaryotes. Initiation of chromosome replication is the first and tightly controlled step of a DNA synthesis. Bacterial chromosome replication is initiated at a single origin, oriC, by the ...
In prokaryotes, the initiator protein DnaA plays an essential role at the initiation step, and feedback control is critical in regulating initiation. Three systems have been identified that exert feedback control in Escherichia coli, all of which are necessary for tight strict regulation of the ...