These suppressor genes help to define those factors that function at particular steps in the initiation pathway. This has always been one of the rate-limiting problems associated with the biochemical analysis of translation initiation, as the complexity of the process was not compatible, in many ...
Synonyms Termination reinitiation mechanism ; Translation reinitiation Definition Reinitiation (REI) is a gene-specific translational control mechanism utilized to down- or upregulate translation of regulatory proteins such as transcription factors and proto-oncogenes in response to various environmental ...
The finding of eIF4A and eIF4G in stress granules suggests that they do not participate in mRNA translation. Human HAP1 cells depleted for eIF2A, eIF2D, or both factors, were able to synthesize luciferase from an mRNA bearing the HCV IRES even when eIF2α was phosphorylated. Overall, ...
Translation initiation in eukaryotes is a complex process involving multiple protein-protein interactions between initiation factors [1]. Cap-dependent translation initiation begins with the recognition of the 5’end of cellular mRNA by the 43S pre-initiation complex (PIC) consisting of 40S ribosomal ...
Throughout all stages of protein synthesis, the ribosome is aided by essential protein co-factors known as translation factors. I have studied the role that two translation initiation factors, IF1 and IF3, play in the mechanism and regulation of translation initiation in Escherichia coli. ...
Translation initiation in human mitochondria relies upon specialized mitoribosomes and initiation factors, mtIF2 and mtIF3, which have diverged from their bacterial counterparts. Here we report two distinct mitochondrial pre-initiation assembly steps involving those factors. Single-particle cryo-EM revealed...
Evolution of Eukaryotic Translation Initiation Factors, Fig. 1 CharacteristicsOf ten eIFs involved in eukaryotic translation initiation, two of them, eIF1A and eIF5B are universally conserved (Fig. 1a ) (for initiation factors, see Translation Initiation and its Table 1). eIF5B is a member of ...
This process is mediated by at least 11 eukaryotic initiation factors and proceeds via the sequential formation of intermediate complexes. Initiation is the rate limiting step of translation, and is a major focus for pathways that regulate gene expression....
In bacteria, translation initiation involves the interaction of the mRNA with the ribosomal small subunit. Additionally, translation initiation factors 1, 2, and 3, and the initiator tRNA, also assemble on the ribosomal small subunit and are essential for efficiently recruiting an mRNA for protein ...
Multiple general transcription factors (GTFs), TBP and TFB, are present in many haloarchaea, and are deemed to accomplish global gene regulation. However, ... Q.,Lu,J.,... - 《Nucleic Acids Research》 被引量: 60发表: 2008年 The TATA-Box-Binding Protein (TBP) of Halobacterium Salinarum...