网络预变性;最初变性;起始加热解开双股螺旋
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FIGURE 1. Generation of a specific PCR product as a result ofaddition of second primer after initial denaturation. Samples were run on a 1.6% agarose gel and a 100 bp DNA ladder (Life TechnologiesGibco BRL) was run in lane 4. Low-molecular-weight non-specificproduct remains the major ...
(dNTPs, MBI Fermentas, Burlington, Canada), 1 μM of each primer, 1 U of Dream Taq Green polymerase (MBI Fermentas, Burlington, Canada) and 50 ng of DNA. Cycling conditions were: an initial denaturation at 95 °C for 5 min, followed by 36 cycles of denaturation at 95 °C for 1 ...
After denaturation of chromosomal DNA with 70% formamide/2 Â SSC at 70 1C, spreads were incubated in 2 Â SSC for 4 min at 70 1C. The hybridization solution (100 ng of denatured probe, 10 mg mlÀ1 dextran sulfate, 2 Â SSC and 50% formamide in a final volume of 30 ml) ...
Innate immune cells quickly infiltrate the site of pathogen entry and not only stave off infection but also initiate antigen presentation and promote adaptive immunity. The recruitment of innate leukocytes has been well studied in the context of extracel
The PCR was performed as follows: one denaturation cycle at 94°C for 5 min, followed by 35 cycles of 94°C for 1 min, 60°C for 1 min and 72°C for 50 s. In the last cycle, the products were kept at 72°C for an additional 5 min. One-fifth of each reaction ...
Denaturation of avian myeloblastosis virus 70 S RNA resulted in the release of one major and several minor species of 4 S RNA which were separated by electrophoresis in polyacrylamide gels. The major 4 S RNA component (one to two molecules per 70 S RNA) was shown to function in the 70 ...
Innate immune cells quickly infiltrate the site of pathogen entry and not only stave off infection but also initiate antigen presentation and promote adaptive immunity. The recruitment of innate leukocytes has been well studied in the context of extracel