A novel method was developed for the precise quantitation of viruses using infrared fluorescent detection of foci of infection in conventional cell culture plates. In this assay, termed the infrared fluorescent immunofocus assay (IR-FIFA), appropriate cell cultures were infected with serial dilutions ...
Here we describe a technology platform to digitally count growth factors in single cells using fluorescent quantum dots (QDs) and calibrated three-dimensional (3D) deconvolution microscopy (QDC-3DM). As a prototypical example, we focus on epidermal growth factor (EGF) and EGF receptor (EGFR)-pos...
β-Glucuronidase (GUS) plays a key role in tumor initiation, metastasis, and progression, and thus, has been proposed as a promising cancer biomarker. In this study, we designed an enzyme-activatable near-infrared (NIR) fluorescent probe (DCM-βGlcA) for
we evaluated the efficacy of NIR-PIT using immunocompetent mice (allograft model) and athymic mice (xenograft model), respectively. Tumor volume curves showed reduced tumor growth in the PIT groups in both mice tumor models. However, the anti-tumor effect of NIR-PIT in allograft models was obs...
(Blue Fluorescent Protein), Calcein, Calcein pH 9.0, Calcium Crimson, Calcium Crimson Ca2+, Calcium Green, Calcium Green-1 Ca2+, Calcium Orange, Calcium Orange Ca2+, Carboxynaphthofluorescein pH 10.0, Cascade Blue, Cascade Blue BSA pH 7.0, Cascade Yellow, Cascade Yellow antibody conjugate pH ...
Progress will be reported on a project to develop a new type of fluorescent immunoprobe containing antibodies conjugated to structurally sorted single-walled carbon nanotubes (SWCNTs). The (,)-sorted SWCNTs display sharp and distinct fluorescence at wavelengths in the short-wave infrared (SWIR) reg...
Mouse breast cancer 4T1 cell line (RRID: CVCL_0125) and 4T1 cell line expressing green fluorescent protein 4T1/GFP/FlucII (RRID: CVCL XG69) were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China) and Tongpai (Shanghai) Biotechnology Co., Ltd. (Shanghai, Chi...
Infrared fluorescent immunofocus assay (IR-FIFA) for the quantitation of non-cytopathic and minimally cytopathic viruses - Counihan, Daniel, et al. - 2006 () Citation Context ...d cells were then washed with MEM and incubated in fresh medium. Infectious HAV was assessed by radioimmunofocus ...
Ching-Hui YangChristopher KasbekHarold A. FiskHumana PressYang C-H, Kasbek C, Fisk HA. 2008. The use of infrared fluorescent dyes in quantitative immunoblotting. In: Walker JM, editor. Pro- tein Protocols Handbook, 3 Edn. London: Humana Press....
ChingHui YangChristopher KasbekHarold A. FiskHumana PressYang C-H, Kasbek C, Fisk HA. The use of infrared fluorescent dyes in quantitative immunoblotting. In: Walker JM, editor. Protein Protocols Handbook. Third Edition ed. London: Humana Press; 2008....