Current the research of transcriptome and proteomics is more about the RNA and protein in cells or immune organs injected by ALV-J to figure out the pathogenic mechanism for the diseases [12]. According to the RNA-seq technology, Lan combined four datasets: ALV gene expression, lncRNA, micro...
RNA purification and quantitative PCR Total RNA from HEK293FT cells was extracted with Trizol reagent (Life Technology). For each sample, 500 ng total RNA was reversed transcripted by ReverTra Ace qPCR RT Master Mix with gDNA Remover Kit (TOYOBO), and 1 μL of cDNA was used for each...