The amplified products were de- tected by gel electrophoresis using the Flashgel System, and 2.2 % agarose gels (Lonza, Basel, Switzerland). The expected product for the Yamagata lineage was 388 bp and 284 bp for the Victoria lineage. Statistical analysis Data analysis was conducted using the...
The system can be a fed-batch system where a culture can be expanded in a single bag from 1 L to 10 L for example, or a perfusion system that allows for the constant supply of nutrients while simultaneously avoiding the accumulation of potentially toxic by-products in the culture medium. ...
Narcolepsy type 1 (NT1) is a rare, chronic brain disorder, characterized by excessive daytime sleepiness, cataplexy and disturbed nocturnal sleep1,2,3. An exceptionally high association of NT1 with the human leukocyte antigen (HLA) class II alleleDQB1*0602, and polymorphisms in the genes encodin...
Here, we test protection against avian flu by using H5N1-derived rHA and GLA-SE, a two-part adjuvant system containing glucopyranosyl lipid adjuvant (GLA), a formulated synthetic Toll-like receptor 4 agonist, and a stable emulsion (SE) of oil in water, which is similar to the best-in-...
simple engineering into multi-domain antibodies and high stability are being exploited for a wide range of applications in biotechnology including diagnostics and immunotherapy31,33, with the first nanobody, Caplicizumab, having been approved for the treatment of a blood clotting disorder in August ...
Public health professionals use 3 criteria to determine if and when a particular disease qualifies as a “public health disorder”: (1) high disease burden affecting many or most members of the population, (2) relatively rapid rate of increase in disease burden, and (3) a sense of fear res...
The polymerase activity of both wild-type Thr339-PB2 and mutant Lys339-PB2 from A/bar-headed goose/Qinghai/15c/2005 (H5N1) was quantitated using a Gluc system in human A549 cells (31). With either wild-type Lys627 or avian-like Glu627, the polymerase activity of Thr339-PB2 was only...
Activation of host innate immune system aims at controlling the spreading and deleterious effects of IAV infection. However, excessive inflammatory response, due to a dysregulation of cytokine release and strong recruitment of neutrophils at the site of infection, may also mediate severe lung inflammatio...
Improved surrogate eggshell incubation system for assisted reproductive technologies such as cloning, genome modification, and de-extinction efforts Hyeon Yang Bo Ram Lee Sung June Byun Scientific Reports (2024) The 3D8 single chain variable fragment protein suppresses Newcastle disease virus transmission...
The cDNA was used as a template for quantitative real-time PCR using TB Green® Premix Ex Taq™ II (Cat#RR820A, Takara, Tokyo, Japan) on an ABI 7500 real-time PCR system. Relative expression levels of the target genes were calculated by the method of 2−∆∆Ct. The primer ...