Lipid nanoparticles (LNPs) have emerged as the predominant delivery vehicle for RNA therapeutics. An important component of LNPs is the ionizable lipid (IL), which is protonated in the acidic environment of the endosome, prompting cargo release into the cytosol. Currently, there is growing ...
Thus, enzymatic hydrolysis of the meals was carried out to increase their solubility, by releasing shorter peptides with more exposed ionizable groups. The hydrolysis reaction was conducted in a jacketed glass reactor of 1 L capacity. Firstly, a solution containing 2.5% of protein w/v was ...
Additionally, transport of ionizable drugs that are initially at high concentrations in liposomes can generate or dissipate pH gradients across the barrier causing deviations from classical pH-permeability profiles. In this study, the liposomal release of a model amine (tyramine) is determined as a ...
The light-scattering data indicated that only a small fraction of the ionizable surface sites (phosphate) of the lipid A-diphosphate was partly dissociated (similar to 30%). It was also discovered that a given amount of Ca2+ (1.0-5.0 nM) or K+ (100 mu M) influenced the structure much...
Usage of a sulfhydryl group in connecting a thiolated antibody with a lipid-containing reactive group, i.e., MAL, is the primary strategy and has been widely studied in the literature. The sulfhydryl group is susceptible to oxidation and can be prevented by substituting oxygen with ...