Lipid nanoparticles (LNPs) have emerged as the predominant delivery vehicle for RNA therapeutics. An important component of LNPs is the ionizable lipid (IL), which is protonated in the acidic environment of the endosome, prompting cargo release into the cytosol. Currently, there is growing ...
Hybrid is based on the separation of ionizable groups into clusters, where the interaction between ionizing charges within a cluster is treated exactly and intercluster interactions are approximated (Gilson, 1993). The atoms of the protein were assigned point charges and radii from the modified ...
the topical application of MB has certain challenges. MB is a cationic and water-soluble molecule. These properties limit its partition and diffusion through the layers of the skin, limiting it from reaching the neoplastic cells at therapeutic...
The results were expressed in mg of tannic acid/100 g of dry matter. 2.1.5.2. Determination of proximate composition The proximate chemical composition of the different flours (moisture, protein, fiber, ash, lipid, carbohydrate contents, and energy value) was carried out according to the ...
Lipid Nanoparticles (LNPs) are used to deliver siRNA and COVID-19 mRNA vaccines. The main factor known to determine their delivery efficiency is the pKa of the LNP containing an ionizable lipid. Herein, we report a method that can predict the LNP pKa from the structure of the ionizable ...
The light-scattering data indicated that only a small fraction of the ionizable surface sites (phosphate) of the lipid A-diphosphate was partly dissociated (similar to 30%). It was also discovered that a given amount of Ca2+ (1.0-5.0 nM) or K+ (100 mu M) influenced the structure much...
Polar ionizable groupsTransition temperatureProtein-lipid interactions at air-liquid interfaces are dependent on electrostatic charges, cations, anions, distribution of protons, and surface potential, which are influenced by pH changes. All of these factors may affect lung surfactant function. To verify...
Thus, enzymatic hydrolysis of the meals was carried out to increase their solubility, by releasing shorter peptides with more exposed ionizable groups. The hydrolysis reaction was conducted in a jacketed glass reactor of 1 L capacity. Firstly, a solution containing 2.5% of protein w/v was ...
ReP1-NCXSQ acquired an orientation in the anionic membranes with the positive end of the macrodipole pointing to the lipid membrane. Potential of mean force calculations, in agreement with experimental measurements, showed that the binding to the anionic interfaces in low ionic strength was ...
Small intestine pH and buffer capacity: implications for dissolution of ionizable compounds... There are more references available in the full text version of this article. Cited by (42) Simulated, biorelevant, clinically relevant or physiologically relevant dissolution media: The hidden role of bicar...