Quantitative RT-PCR The qRT-PCR for SARS-CoV-2 was assessed as described previously.13 Nasopharyngeal specimens collected during hospitalization were sent to the laboratory in a viral transport case. Total nucleic acid extraction from the samples was performed using the QIAamp RNA Viral Kit (Qiagen...
Viral hemorrhagic septicemia (VHS) is a cold-water disease caused by viral hemorrhagic septicemia virus (VHSV) at an optimal temperature of 9 degrees C-15 degrees C. VHSV isolation and detection have been accomplished by using a number of diagnostic methods such as cell culture and qRT-PCR. ...
Recently, microRNA (miRNA) as a new disease diagnosis biomarker has been intensively studied in many areas, such as various cancers, heart disease, pregnancy, diabetes, psychosis, and various infectious diseases [9–15]. Studies have shown that miR-155 and miR-155* in peripheral blood mononucle...
使用物种特定的实时PCR分析法检测感兴趣的动物。 作者:Pantchev A.;Sting R.;Bauerfeind R.;Tyczka J.;Sachse K. 期刊名称:《Comparative Immunology, Microbiology and Infectious Diseases》 | 2010年第6期 关键词: bacterial diseases; detection; diagnosis; diagnostic techniques; disease prevalence; disease...
disease has to be differentiated from pancreas disease (PD) caused by salmonalphavirus, which also affects skeletal and heart muscle. Confirmatory diagnosis of IPN can easily be done by virus isolation and RT-PCR. IPN was removed from the OIE list of notifiable aquatic animal diseases in 2005 ...
Changes of miRNAs in exosome have been reported in different disease diagnosis and provided as potential biomarkers. In this study, we compared microRNA profile in exosomes in 5 MHFMD and 5 ESHFMD as well as in 5 healthy children. Different expression of
To investigate the potential molecular mechanism in the radiation effects on hydatid disease, we determined the expression of related genes using real-time PCR. TPX is expressed in all developmental stages of E. granulosus, which is of great importance in repairing the injury induced by reactive ox...
and types of CRISPR-Cas as the basis for the emerging CRISPR-diagnostic platforms, focusing on CRISPR-Cas systems already used for molecular diagnosis, and describe their properties, functions, and perspectives to become the ideal platforms for diagnosing infectious diseases and curbing disease ...
SARS-CoV-2 is an enveloped virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. Here, single viruses were analyzed by atomic force microscopy (AFM) operating directly in a level 3 biosafety (BSL3) facility, which appeared as a fast and powerful method to assess at the na...
Using anti-HSV-1 (Gg1) IgM and anti-HSV-1 (gG1) IgG Euroimmun and the detection and quantification of HSV-1 viral load in plasma samples, through real-time PCR. The maps were constructed using QGIS and the statistical analyses using R Studio software. Results A total of 1138 individuals...