目的:研究INF-γ通过ATGL诱导肝癌细胞HepG2凋亡的作用及分子机制.方法:ELISA法检测血清中INF-γ水平;MTT法检测肝癌细胞HepG2活力;Caspase-3酶活性试剂盒检测Caspase-3酶活性;过表达ATGL,研究INF-γ通过ATGL诱导肝癌细胞HepG2凋亡作用.结果:肝癌病人血清中INF-γ水平明显降低(P < 0.001);INF-γ通过ATGL诱导肝癌细胞...
Levels of IFN-gamma and IL-27 in serum and pleural effusion were detected by enzyme-linked immunosorbent assay (ELISA). Relative expression of IFN-gamma mRNA and IL-27 mRNA in peripheral blood mononuclear cells were detected by RT-PCR. Positive rate of T-Spot TB in observation group was ...
| progen | BOC | 进口现货产品 | 胎牛血清 | 细胞培养 | 提取试剂 | 转染试剂 | 常规生化试剂 | ELISA试剂盒 | 抗体 | 细胞因子 | sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVisi...
Levels of inflammatory factors were measured using high-sensitivity photon-emission based ELISA. At baseline, no differences were found for inflammatory factors between rehabilitation patients and controls nor between diagnostic groups. Repeated measures ANOVAs showed that IL-6 levels decreased over time....
The mRNA and protein expression levels of INF-gamma were assessed by real-time RT-PCR and ELISA, respectively. The histone acetylation status of the INF-gamma promoter and the binding of RNA polymerase II (RNA Pol II) to the INF-gamma promoter were detected using a chromatin ...
| progen | BOC | 进口现货产品 | 胎牛血清 | 细胞培养 | 提取试剂 | 转染试剂 | 常规生化试剂 | ELISA试剂盒 | 抗体 | 细胞因子 | sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVisi...
Furthermore, the authors have studied the IL-12-induced expression of the T H1-associated cytokine INF-纬 and the IL-4-induced expression of the T H2-specific cytokine IL-5 in the presence of LPS using ELISA and compared nine atopic asthmatic subjects and eleven nonatopic normal volunteers....
| progen | BOC | 进口现货产品 | 胎牛血清 | 细胞培养 | 提取试剂 | 转染试剂 | 常规生化试剂 | ELISA试剂盒 | 抗体 | 细胞因子 | sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVisi...
Production and mRNA levels of pro-inflammatory chemokines and cytokines were assessed using commercial ELISA kits and qRT-PCR analysis, respectively [1]. HaCaT cells were plated in 60 mm culture dishes at a density of 3.5 × 105 cells per dish and incubated for 48 h. They were then treated...