RESULTS. We developed a Python module called MSModDetector that studies PTM patterns from individual ion mass spectrometry (I2MS) data. I2MS is an intact protein mass spectrometry approach that generates true mass spectra without the need to infer charge states. The algorithm first detects and ...
LCMS grade water, isopropanol, and acetic acid were purchased through Fisher Scientific. All samples were separated using a Sciex ExionLC™ AC system and measured using a Sciex 5500 QTRAP® mass spectrometer. Aqueous metabolites were analyzed using a previously established ion pairing method...
In contrast, the size mode of the particles generated from isotopically labeled 13C graphite electrodes peaked in the accumulation mode, with 73% of the particles producing EC carbon ion cluster patterns at m/z 13 (13C+), 26(13C2+), and 39 (13C3+), with additional organic carbon ...
Lipid quantification by shotgun mass spectrometry Mass spectrometric analyses were performed on a Q Exactive instrument (Thermo Fisher Scientific, Bremen, Germany) equipped with a robotic nanoflow ion source TriVersa NanoMate (Advion BioSciences, Ithaca NY, USA) using nanoelectrospray chips with the di...
To determine whether the C-terminus of APJ is phosphorylated in an agonist-dependent manner, we performed mass spectrometry. Specifically, we stimulated HEK293 cells stably transfected with APJ with five different peptides: apelin-36, apelin-17, apelin-13, ELA-32, and ELA-21. The five peptide...
An apparatus for counting individual particles in time-of-flight mass spectrometry includes a device for producing secondary particles at a high repetition rate by pulsed ion bombardment of a sample from which the secondary particles originate, an accelerator for accelerating the secondary particles, and...
electrospray ion beam deposition, which was developed by a team headed by Stephan Rauschenbach in Klaus Kern's department. The researchers expose the protein solution to a high electrical voltage so that the liquid is highly charged. Electrical repulsion then causes the liquid to atomize into a fi...
Electrospray ionization mass spectrometry (ESI-MS) has evolved to be one of the most powerful technologies for quantitative analyses of individual lipid species. Selected ion monitoring (SIM) is an MS scanning mode in which only a single m/z or a limited number of m/z ratios is transmitted ...
(and final polishing at 2 kV) using a Thermo Fisher Scientific G4 DualBeam UX Focused Ion Beam (FIB) analogous to the procedure described in ref.42. All APT needles were prepared with thec-axis along the needle axis as this gives the optimal spatial resolution due to the layer-by-...
c, EIC for m/z 202.1085 (negative ion mode), corresponding to C9H16NO4-, from exo-metabolome extracts of N2 and the three wild strains. d, MS/MS fragmentation for metabolites A-F in (c). Metabolites A and B have near-identical MS/MS spectra, that differ significantly from the near...