Affinity purification/immunoblotting Cells were harvested and lysed in 0.5% digitonin, 75 mM Tris (pH 8.0), 2 mM EDTA, 5 mM MgCl2, 1 mM DTT with protease and phosphatase inhibitors. Cleared supernatants were then subjected to affinity purification with streptavidin sepharose (GE Healthcare, ...
Quantification of protein bands was performed for each sample by determining the relative optical density (ImageJ; National Institutes of Health, Bethesda, MD). Data processing MS/MS spectra were extracted from raw files requiring a minimum of 50 signals with an intensity of at least 1 x 105 ...
Infectious diseases are a major concern of public health because of the potentially high mortality and financial costs to health systems1. To avoid or reduce the impact of an epidemic outbreak, it is necessary to understand the mechanisms driving the spreading dynamics. The population dynamics of ...
especially in the association cortex. Research has demonstrated a link between early socioeconomic status (SES) and variations in both brain structure and function, which are further associated with cognitive and mental health outcomes. However, the extent to which SES is associated...
Rectal preparations have the advantage of being easy to administer in rural areas; therefore it is anticipated that rectal administration of an artemisinin derivative in remote settings might "buy time" by halting or slowing the progress of disease while a patient is being transported to a health-...
Electronic medical record (EMR) is currently a popular topic in e-health. EMR includes the health-related information of patients and forms the main factor
Li Li1,Yi‑fan He1, Miao‑miao Guo1, Jing Li2, Xiao‑hui Zhang2, Zhi Lu3, Jie Gu3, Jing‑lin Bao3 & Hong Meng1 Geographical, environmental and pollution conditions affect facial skin health, but their effects on skin appearance have not been elucidated. ...
Jun Zhou, Qiang Wen, Binbin Xu, Bing Qi, Jie Gao, Hongmeng Li, Linjing Jia & Hailing Qiao The lack of information concerning individual variation in content and activity of human liver microsomal protein is one of the most important obstacles for designing personalized medicin...
Acknowledgements This research was directly supported by the Intramural Research Program of the National Institutes of Health (NIH), NINDS, grants NIH HL36783 to D.C.G., and NIH U54GM087519 and R01GM030376 to F.B. We thank the Section on Instrumentation of the National Institute of ...
PACUC ensures that all ani- mal programs, procedures, and facilities at Purdue University adhere to the policies, recommendations, guide- lines, and regulations of the USDA and the United States Public Health Service (USPHS) in accordance with the Awnasimcoanl fWiremlfeadrebAycctearnvdicaPludrid...