We initially modified and optimised a human indirect immunofluorescence assay (IFA) test for the detection of IgG antibodies against phase I and/or phase II Coxiella burnetii in bovine sera and determined an optimal screening dilution cut-off to be 1:160. Direct comparison of the modified IFA ...
B, optimal incubation time after ASFV infection to perform the immunofluorescence assay (IFA). IFA was performed using ASFV positive and negative sera at different hours post-infection (hpi). To determine the optimal infection time, BK2258 cells were infected with ASFV SD/DY-I/21 and cultured ...
Immunofluorescence assay (IFA) is astandard virologic technique to identify the presence of antibodies by their specific ability to react with viral antigens expressed in infected cells; bound antibodies are visualized by incubation with fluorescently labeled antihuman antibody. What is indirect haemaggluti...
(IFA) Les tests d’immunofluorescence indirecte (IFA) jouent un rôle clé dans la détection et la titration des auto-anticorps. Ils associent les principes immunologiques à la microscopie à fluorescence pour fournir des résultats hautement spécifiques et sensibles. L’immunofluorescence ...
Indirect immunofluorescence assay Anti-SARS-CoV-2 anti- bodies were detected using an in-house indirect immunofluo- rescence assay (IFA), as previously described [9]. Vero E6 cells (ATCC CRL-1586, Rockville, MD, USA) infected with the SARS-CoV2 strain IHU-MI2 (full genome sequence of ...
Objective: To evaluate the value of indirect immunofluorescence assay (IFA) in the diagnosis of genital herpes. 目的:评价间接免疫荧光试验(IFA)在生殖器疱疹(GH)诊断中的应用价值。 3) IFA 间接免疫荧光试验 1. Then the recombinants were transfected into COS-7, it was proved that the env gene wer...
The present investigation was carried out to detect Nocardiosis in immunocompromised patients confined in the pulmonary ward of through the use of indirect immunofluorescence assay (IFA) and bacterial culture methods. The comparison of the two methods and the correlation between the antibody titer and ...
将接种PCV2且经多次传代的PK-15带毒细胞分散到96孔细胞培养板上,制成抗原诊断板,应用间接免疫荧光技术检测血清中PCV2的抗体。 更多例句>> 4) indirect immunofluorescence technique 间接荧光免疫技术 5) PPA ELISA 免疫荧光抗体诊断技术 1. The samples that are suspicious of hog cholera virus infected were det...
Comparison between theindirect immunofluorescence antibody (IFA) and dot blot enzyme immunoassay (EIA) methods is reported.Materials and Methods: A commercial dotblotimmunoassay, ImmunoDOTTM Coxiella Burnetii, wasdeveloped using a cell-free culture method to preparepurified phase I and II Coxiella ...
1) indirect immunofluorescence antibody technique 间接荧光抗体技术1. Thirty eight serum samples from dogs vaccinated with attenuated caine distemper virus (CDV) were detected by serum neutralization (SN) assay, indirect immunofluorescence antibody technique (IFAT) and immunohistochemical technique, respectively...