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The stained samples were visualized under a confocal microscope (Olympus Fluoview TM-FV1000, Olympus Life Sciences, Tokyo, Japan) with a 63× oil immersion objective, at excitation of 400–490 nm, emission ≥ 520 nm. Fluoview FV10 software, ver 1.7 (Olympus Life Sciences, Tokyo, Japan) was...