Cells were grown on coverslips with water-shedding coating (Matsunami, Kishiwada, Japan). Purified IgG (0.5 mg/mL) from patients was applied to the culture media, and the cells were incubated for 30 min at room temperature. Next, the cells were fixed in 4% paraformaldehyde for 30 min, per...
The Electric Zine Maker captures the love of DIY culture and zine making, distilling its values into a colorful space that lets you explore your creative whims. The Guardian called the Electric Zine Maker "a clever, accessible tool in the disguise of a joyful toy" and it was described as ...
这也就是我们应该继续保留的,也是我们 Through from the national culture inquired about the Chinese animation the artistic style, enables the Chinese animation both the maintenance independence and to have the innovation in the world animation development.This also is we should continue to retain, also...
To forming the Pogues and the conquering the known universe, we discover MacGowan’s passions, his humor and deep knowledge of music, history, spirituality & popular culture. For this is Shane’s story. A vision of the world through the eyes of the great punk poet himself and an intimate...
the ‘rules.’ In Japan, those properties belong to regional or federal governments, who are usually very difficult to challenge, while in places like Paris, for instance, such public screenings happen all the time. We need our government to be much more supportive to the filmmaking culture....
* o·ta·ku — noun (in Japan) a young person who is obsessed with computers or particular aspects of popular culture to the detriment of their social skills. Some Things Five things that have been important and influential in my life by Takashi Murakami: Galaxy Express 999 (manga ...
We are pleased to announce the remainder of the SXSW Film Festival program including the Midnighters, Shorts, Virtual Cinema, Music Video, Title Sequence and new Independent Episodic lineup, plus late-addition Features and the Closing Night Film for the
From infant formula, clothing Toys, maternity products to education and training, cultural animation, baby industry almost everything. Like early electrical appliances, cars, etc. industry In general, "foreign brands" hold the absolute initiative in the baby market. Japan, Australia's foreign milk ...
celebration of creativity, culture, and community Nico is a passionate filmmaker and the visionary founder of the New York State International Film Festival, which he established in Albany, NY, in 2016. With a deep love for storytelling and creating new productions, Nico has dedicated his career...