Polymerase chain reaction (PCR) is an enzymatic reaction whose efficiency and sensitivity largely depend on the efficiency of the primers that are used for the amplification of a concerned gene/DNA fragment. Selective amplification of nucleic acid molecules initially present in minute quantities ...
DNA amplification with polymerase chain reaction (PCR) is a ubiquitous protocol that scientists across academia and the industry know well. (You may even have been introduced toPCR in songonce upon a time.) In a typical PCR protocol, the first step is denaturation, to separate the template DN...
摘要: A successful polymerase chain reaction (PCR) is the consequence of efficient primer designing and selective amplification of the target genetic region. The advancement of computational algorithms has...DOI: 10.1007/978-3-030-02634-9_16 年份: 2019 ...
In silico PCR analysis is a useful and efficient complementary method to ensure primer specificity for an extensive range of PCR applications from gene discovery, molecular diagnosis, and pathogen detection to forensic DNA typing. In silico PCR, SNPCheck, and Primer-BLAST are commonly used web-based...
Theoretical PCR amplification, AFLP-PCR and restriction digest of complete bacterial genomes. genomic data from sequenced prokaryotes.
摘要: In silico PCR analysis is a useful and efficient complementary method to ensure primer specificity for an extensive range of PCR applications from gene discovery, molecular diagnosis, and pathogen...关键词: Polymerase chain reaction primer binding site single nucleotide polymorphism (SNP specific...
This chapter introduces the software FastPCR as an integrated tools environment for PCR primer and probe design. It also predicts oligonucleotide properties based ...
In-silico PCR for a set of primers Dicey can search for multiple primer pairs, show off-target products and determine PCR amplicons. echo -e ">FGA_f\nGCCCCATAGGTTTTGAACTCA\n>FGA_r\nTGATTTGTCTGTAATTGCCAGC" > primers.fa dicey search -c 45 -g hg19.fa.gz primers.fa | python script...
new module: crabs/insilicopcr Is there an existing module for this? I have searched for the existing module Is there an open PR for this? I have searched for existing PRs Is there an open issue for this? I have searched for existing issues...
4th column : When the quadruplex PCR give an ambiguity, this column show the alleles specific for groups C and E. 5th column : final conclusion about phylogroup. Citing Please cite: Beghain, J., Bridier-Nahmias, A., Le Nagard, H., Denamur, E. & Clermont, O. ClermonTyping: an eas...