In-line probing assay of a Moco RNAElizabeth E RegulskiRyan H MoyZasha WeinbergJeffrey E BarrickZizhen YaoWalter L RuzzoRonald R Breaker
Regulski, E.E., Breaker, R.R. (2008). In-Line Probing Analysis of Riboswitches. In: Wilusz, J. (eds) Post-Transcriptional Gene Regulation. Methods In Molecular Biology™, vol 419. Humana Press. https://doi.org/10.1007/978-1-59745-033-1_4 Download citation .RIS .BIB DOIhttps://...
CellTitre 96® AQueous One Solution Cell Proliferation Assay was supplied by Promega (Madison, WI, USA). Caco-2 cells (passage 26–32) were obtained from the European Collection of Cell Cultures (Salisbury, UK). HepG2 (34–40) cells were obtained from the American Type Culture Collection...
Apoptosis was also evaluated by the ApoONE Homogenous Caspase 3/7 Assay. A spectrofluorometer 96 wells plate reader (Wallac VICTOR2, Turku, Finland) was used for measuring the fluorescence of 5×104 cells/well of both HL60/LXSN and HL60/HOXB1. Cells were kept in 1% FBS or in 10% FBS...
Similar results were obtained using bovine microtubule fractions and probing with both Tau-nY197 and Tau-nY394 antibodies (data not shown). Download: Download high-res image (262KB) Download: Download full-size image Figure 4. Tau-nY197 labels tau from nonprimate species. A: Tau protein ...
The present study was performed to verify that the exogenous application of proline as an antioxidant can effectively reduce the damage of the herbicide Basagran® on fenugreek (Trigonella foenum-graecum). Sterilized healthy seeds were soaked in proline (7 mM) with or without the herbicide Basa...
Phospholipid concentration was determined to <1% error using inorganic phosphate assay [27]. Fluorescent dyes were TopFluor-PC, here named TFPC (1-palmitoyl-2-(dipyrrometheneboron difluoride) undecanoyl-sn-glycero-3-phosphocholine) from Avanti Polar Lipids (Alabaster, AL, USA); DiD (1,1′-...
The results indicated that cinobufagin could decrease the cell viability, increase the apoptosis proportion and reverse the cisplatin resistance in SGC7901-DDP cell line by protein kinase B/mammalian target of rapamycin signal pathway. Lastly, the in vitro assay was completed and the result ...
Apoptosis was also evaluated by the ApoONE Homogenous Caspase 3/7 Assay. A spectrofluorometer 96 wells plate reader (Wallac VICTOR2, Turku, Finland) was used for measuring the fluorescence of 5×104 cells/well of both HL60/LXSN and HL60/HOXB1. Cells were kept in 1% FBS or in 10% FBS...
Results: The AGE rich extract BCE induced the NF-kB pathway in macrophage cells and also showed a concentration-dependent antioxidative capacity by the DPPH-assay. With the LC/MS and amino acid analyses, we identified the presence of the protein gliadin in BCE confirmed by using specific ...