Various in vitro, ex vivo and in vivo models exist, each associated with defined advantages and disadvantages. In vitro and ex vivo models include standard, genetically modified and patient-derived cell lines, spheroid and organoid culture models, scaffold and hydrogel cultures, and tissue slice mod...
To this aim, in vitro and ex vivo angiogenesis assays may be suitable for a rapid screening of potential anti-angiogenic molecules before in vivo validation of the putative lead compounds. This review focuses on the different in vitro and ex vivo angiogenesis assays that have been developed over...
In vitro, the maximal bactericidal activity was obtained with amoxycillin (1 x MIC versus 2 x MIC cefpodoxime and 4 x MIC cefuroxime). Mice were challenged by intraperitoneal inoculation and treated orally every 8 h for 48 h with 2.5, 5, 7.5 and 10 mg/kg doses of these three beta-...
In vivo and ex vivo methods of growing a liver bud through tissue connection Cell-based therapy has been proposed as an alternative to orthotopic liver transplantation. The novel transplantation of anin vitro-generated liver bud might have therapeutic potential.In vivoandex vivomethods for growing a...
www.nature.com/scientificreports OPEN Depurinized milk downregulates rat thymus MyD88/Akt/p38 function, NF-κB-mediated inflammation, received: 26 September 2016 caspase-1 activity but not theaccepted:04January2017 endonuclease pathway:Published:08February2017 in vitro/in vivo study Gordana Kocic1...
Ex vivo (tissue-slices) • Complex architecture• Capability for functional tests used on 2D slices• Contains all cell types • Loss of tissue function• Short-term culture only• Low access to human tissue (Hansen et al., 2010; Morrison et al., 2006) 2D in vitro • Inexpensiv...
(P)H. In contrast to the MFCs, the changes in the metabolic state of hepatocytes in the ex vivo models are, to a greater extent, associated with compensatory processes. Thus, MFCs in combination with FLIM can be applied as an effective tool set for the express modeling and diagnosis of ...
lining and to load it with a defined quantity of dexamethasone, first to evaluate the release kinetics in vitro and then ex vivo on human corneas stored in a bioreactor. Finally, we compared the drug penetration in corneal tissue from the loaded biomaterial versus commercial dexamethasone eye ...
Among tested models, the largest number of compounds was found within the in vitro 2D cell culture model, while in vivo and in vitro 3D models had the lowest number of detected compounds. These results may be connected with a higher metabolic rate, as well as lower integrity of the in ...
GT-1 contains a siderophore fused to a novel cephalosporin to create such a molecule. Of the panel of nosocomial and community-acquired bacterial pathogens tested, the combination of GT-1 and a broad-spectrum BLI, GT-055, demonstrated the greatest in vitro efficacy versusE. coliandE. cloacae,...