Dolznig, In vitro cell migration and invasion assays, Mut. Res. 752 (2013) 10e24.Kramer N, Walzl A, Unger C, Rosner M, Krupitza G, Hengstschlager M, Dolznig H. In vitro cell migration and invasion assays. Mutat Res 2013; 752:10-24; PMID:22940039; http://dx.doi.org/10.1016/j....
We first characterized the impact of triggering calcium signals in eOS1-expressing B3Z T cell hybridoma in vitro. B3Z cells exhibited typical ameboid migration on Poly-L-Lysine (PLL) + ICAM-1-coated surfaces. Upon photoactivation, most B3Z cells immediately stopped and rounded up (Fig. ...
Cell migration Cell invasion Matrigel Techniques Cell Migration Assay Cell Invasion Assay Cell And Tissue Culture Reagents Please check back later as we are actively working on gathering the necessary information. Thank you for your understanding and patience. ...
Fig. 1: Low U1 AMO dose-dependently enhances migration and invasion of HeLa cells in vitro. a HeLa cells were transfected with the indicated amounts of U1 or control AMOs. Cell proliferation was determined from cell numbers using the Cell Titer-Glo Luminescent Cell Viability Assay at the times...
Cell migration is a key feature of virtually every biological process, and it can be studied in a variety of ways. Here we outline a protocol for the in vitro study of cell migration using a ring barrier–based assay. A 'barrier' is inserted in the culture chamber, which prevents cells ...
Y Yanagi,K Nakayama,T Taguchi,... - 《Sci Rep》 被引量: 10发表: 2017年 [Methods in Molecular Biology] T-Cell Trafficking Volume 1591 || In Vitro and Ex Vivo Models to Study T Cell Migration Through the Human Liver Parenchyma Cell-based therapy has been proposed as an alternative to ...
Transwell® 6.5 mm inserts with 8.0 μm pore polycarbonate membranes (Corning, NY, USA) and Cell Invasion Kits (Millipore, Billerica, MA) were used for the Transwell cell migration/invasion assay. Ovarian cancer cells were resuspended in FBS-free medium and seeded in the Transwell cham...
We further explored the effects ofKITLG/KITpathway activation in MCs on the proliferation and migration of CRC cells through in vitro experiments. Initially, PCR and Western Blot analyses were performed to assessKITexpression in the P815 cell line after 48 h of co-culturing withKITLG. Both assa...
opening the door to advanced T cell therapies in preclinical and clinical trials. CRISPR–Cas9 can edit both primary T cells and engineered T cells, including CAR-T and TCR-T, in vivo and in vitro to regulate T cell differentiation and activation states. This review first provides a comprehen...
PRL-3 is highly expressed in patient T-ALL samples at both the mRNA and protein levels compared to normal lymphocytes. Knock-down of PRL-3 expression using short-hairpin RNA (shRNA) in human T-ALL cell lines significantly impeded T-ALL cell migration capacity in vitro and reduced their ...