In vitro study of human skeletal muscle strips: effect of nonesterified fatty acid supply on glucose storage. Metabolism. 1989 Dec; 38 (12):1183–1187.Argyraki M, Wright PD, Venables CW, Proud G, Taylor R (1989) In vitro study of human skeletal muscle strips: effect of nonesterified ...
ABX-erased sex-difference in glucose metabolism was mainly due to the improvement of male mice in response to gut microbiome depletion (Fig.2f–j). Further, the pAkt levels in the
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The strips were incubated in trypsin/EDTA at 37 °C for 8–10 min. The cells were centrifuged (300× g, 5 min), seeded into a 6-well tissue-culture-treated polystyrene (TCPS) plate and cultured in low-glucose DMEM supplemented with 10% FBS and 1% penicillin-streptomycin in a...
Furthermore, it can be formulated into wearable or disposable strips, which eases application. On the other hand, this system also has a few disadvantages such as difficulty in sweat collection, temperature changes and activity variation of glucose oxidase. Enzyme stability is also a challenge with...
Pathak and Dhawan (2012b) found that the growth of rootstock M7 and MM111 shoots could be supported on medium containing between 1 and 5% fructose, glucose, mannitol, sorbitol and sucrose, with the most effective concentration resulting in longest shoots and greatest productivity arising from 4%...
The in vitro subtractive screening proposed in this work also include the assessment of bile salts deconjugation, mucin degradation, biogenic amine production and other potentially detrimental enzymatic activities such as the β-glucuronidase activity, which should be absent in probiotic candidates [54–56...
“Warburg effect” whereby cells increase their lactate output to fuel their ever-increasing metabolic demands, we determined the metabolic state of undifferentiated stem cells before and after treatment with a myogenic stimulus in vitro. The levels of glucose and glutamine consumption in addition to ...
USA) with the median value of 5 measurements recorded. Glucose and insulin tolerance testing was performed after 16 or 6 h fasting and i.p. injection of 2.5 mg glucose or 0.75 mU of insulin/g body weight, respectively. Glycemia was measured using Accu-Check Aviva glucostrips. Whole...
In non-diabetic β-cells, glucose uptake and its subsequent phosphorylation by glucokinase drive glycolysis and pyruvate generation10. Pyruvate enters mitochondria, where it is oxidised in the tricarboxylic acid (TCA) cycle, generating reducing equivalents (NADH and FADH2) that are utilised by the ...