Cell-free in vitro replication assay (IVRA) is one of the widely used assays to understand the complex mammalian replication system. IVRA can provide a snapshot of the regulatory mechanisms controlling replication in higher eukaryotes by using a single plasmid, pEPI-1. This chapter outlines the ...
Fig. 3: In vitro replication of a 116.3 kb multipartite DNA genome. a The collection of 11 plasmids (combined length 116.3 kb), which were co-replicated in PURErep. In addition to Phi29-DNAP (pREP, 6 nM input), the plasmid ensemble encodes the ribosomal rRNA operon rrnB (prRNA...
Moreover, as a cell culture system for studying HBV, the viral replication level in the HepaRG cell line is far less than that obtained with plasmid transfection. These issues have created bottlenecks for current in vitro infection experiments. Because this cell line can express various ...
Allogeneic peripheral blood NK cells have been shown to efficiently inhibit viral replication in vitro24 and acute HIV infection in a humanized mouse model25. Recently, activated NK cells primed with IL-15 inhibited latently infected cells from propagating infection in vitro26, suggesting NK cells ...
Some ICE and ICE-like elements, however, have been reported to be capable of autonomous replication [53–57]. In the light F plasmid-mediated mobilization of the HPI [13], it would, nevertheless, also be interesting to analyse in the future if a PAI II536 construct, which is not a ...
Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of both polarities of the genomic RNA was detectable 24 h post inoculation. ...
Lipid-disrupting oral rinses reduce viral infectivity in vitro achieving EN14476 virucidal standards Having characterized the envelope composition, we next investigated whether it was possible to disrupt the lipid envelope using oral rinses that not only have been designed to be antimicrobial but also co...
In conclusion, siRNA/VIPER polyplexes efficiently delivered siRNA to lung epithelial cells and mediated robust downregulation of viral replication both in vitro and ex vivo without toxic or immunogenic side effects in vivo, demonstrating the potential of local siRNA delivery as a promising antiviral ...
[210].In vitroandin vivostudies showed efficient bypass of BaP-G by TLS Pol κ using a gapped plasmid containing a BaP-G lesion [211]. Additionally, BaP-G has been shown to be bypassed by a two-polymerase mechanismin vivo. TLS Pol κ inserts nucleotides opposite a BaP-G error-free ...
GFP-microtubule-associated protein 1 light chain 3 (GFP-LC3) plasmid was a gift from Prof. Songshu Meng (Dalian Medical University, China). Hormone-resistant human prostate cancer cell PC3 cell line was purchased from the Cell Bank of the Chinese Academy of Science (Shanghai, China) and pre...