Equilibrium binding of estradiol by uterine cell suspensions and whole uteri in vitro. Biochemistry. 1974 Dec 31; 13 (27):5537–5542. [ PubMed ]Williams D., Gorski J. : Equilibrium Binding of Estradiol by Uterine Cell Suspensions ... ...
Stimulation was given as percent of the specific [35S]GTPγS binding observed over the basal activity, which was settled as 100%. In the competition binding assays the ‘One site competition’ fitting was used to establish the equilibrium binding affinity (Ki value). Inhibition was given as ...
In Vitro Assessment of Plasma Protein Binding of 20(R)-Ginsenoside Rh2 by Equilibrium Dialysis and LC-MS Analysis : A Case of Species Differences(Pharmacol... et al.In vitro assessment of plasma protein binding of 20(R)-ginsenoside Rh2 by equilibrium dialysis and LC-MS analysis:a case of ...
Since the translational modification is reversible, the dynamic equilibrium of acetylation and deacetylation, methylation and demethylation are important for maintaining the activity of STAT3. As shown in Fig.1a, the acetylated STAT3 at Lys615, Lys631 and Lys685 are deacetylated and deacetyliminated b...
RESULTS AND CONCLUSION Compared with equilibrium dialysis and ultrafiltration method, microdialysis,a new technique for the in vitro or in vivo sampling has significant advantages and broad prospects in the study of drug-protein binding. Key words: microdilysis technique drug-protein binding influential...
levels protect against it [11]. Additionally, when cysteine is scarce, gamma-cysteine ligase's catalytic unit (GCLC) activates a GSH-independent defense by utilizing an alternative amino acid to prevent ferroptosis, highlighting a non-traditional pathway for maintaining antioxidant system equilibrium [...
C. Equilibrium free-energy differences from a linear nonequilibrium equality. Phys. Rev. E 103, 032146 (2021). ADS MathSciNet CAS PubMed Google Scholar Kabsch, W. & Sander, C. Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. ...
Concentrations of non-micellar (protein-bound plus unbound) docetaxel were estimated from the measured unbound concentrations and percent binding data from the in vitro binding study; however, the non-micellar pool of docetaxel present in the plasma after micellar docetaxel administration was almost ...
The study of Aβ aggregation and antibodies that bind to Aβ aggregates is complicated by the fact that multiple Aβ species exist in a dynamic equilibrium6,51. In order to produce soluble aggregates of Aβ free of both Aβ monomer and fibrils, we used a covalently-stabilized synthetic ...
Metabolism controls gene expression through allosteric interactions between metabolites and transcription factors. These interactions are usually measured with in vitro assays, but there are no methods to identify them at a genome-scale in vivo. Here we