We have used this approach with a satellite RNA of cucumber mosaic virus (CMV) that is replicated only in CMV-infected cells and attenuates the symptoms induced by CMV. The cDNA copy of CMV satellite RNA was in
Virus-like particles are an emerging class of nano-biotechnology with the Tobacco Mosaic Virus (TMV) having found a wide range of applications in imaging, drug delivery, and vaccine development. TMV is typically produced in planta, and, as an RNA virus, is highly susceptible to natural mutation...
Plant stress is an unfavourable environmental constraint affecting the plant growth and development leading to a worldwide loss in agricultural productivity. The environmental stress on plants can be biotic or abiotic. Biotic stress involves the damage c
heavy-metal stress of mercury by synthesizing phytochelatins (PCs) that act as chelators. The strength of Hg(II) binding to glutathione and phytochelatins follows the order: γGlu-Cys-Gly(γGlu-Cys)2Gly(γGlu-Cys)3Gly(γGlu-Cys)4Gly. Suspension cultures of haploid tobacco,Nicotiana tabacum...
Encephalomyocarditis virus eYFP: Enhanced yellow fluorescent protein FFLuc: Firefly luciferase ICE: Insect cell extract IMAC: Immobilized metal-affinity chromatography RLL: Rabbit reticulocyte lysate RRLuc: Renilla reniformis luciferase SEC: Size exclusion chromatography TMV: Tobacco mosaic virus ...
A 301-bp fragment of each gene was placed in a tandem in the Tobacco rattle virus (TRV)-based vector downstream of a 300 bp fragment of Phytoene Desaturase (PDS), silencing of which was used as a visual marker for VIGS effectiveness. PDS silencing alone did not affect the benzaldehyde ...
Large-scale performance of transgenic tobacco plants resistant to both tobacco mosaic virus and cucumber mosaic virus D.D. Jones (Ed.), Proceedings of the Third International Symposium on the Biosafety Results of Field Tests of Genetically Modified Plants and Micro-organisms, University of California...
Step-by-Step Solution:1. Understanding Genetic Material: - Genetic material in organisms can be either DNA (deoxyribonucleic acid) or RNA (ribonucleic acid). 2. Identifying RNA Viruses</stron
Thus far, only Tobacco mosaic virus (TMV) has been synthesized de novo, although the initial synthetic genome was not infectious due to the presence of errors in the original sequence deposited in 1982 [27]. The authors aligned the original sequence with more recent strains and identified the ...
In vivo, the generation of viruses by the injection of nucleic acids into the cells of living organisms was first studied following the discovery of the infectious power of Tobacco mosaic virus RNA by Gierer and Schramm in 1956 [Citation8]. Subsequently, advances in genetic engineering have enabl...