The coding sequence ofM. hrabeiOR5 (MhOR5) was synthesized as a gene fragment (Twist). Residues Lys2 to Pro474 were cloned into a pEG BacMam vector37containing N-terminal tags of Strep II, superfolder GFP38, and an HRV 3C protease site for cleavage (N-CACCatg-ST2-SGR-sfGFP-PPX-AscI-...
To this end, the gene expression profile was investigated and compared between Jin668 and TM-1 in the same cell type, at the same induction time, to further understand cell fate transitions into embryonic development. The results reveal multiple DEGs in different cells. The DEGs in epidermis (...
This could be considered as an example for successful use of morphological markers to modern breeding. In wheat breeding, the dwarfism governed by gene Rht10 was introgressed into Taigu nuclear male-sterile wheat by backcrossing, and a tight linkage was generated between Rht10 and the male-...
If the gene of interest was cloned at Pvu I site in pBR322 ,the recombinant bacteria would exhibit resistance to which drug ? View Solution If the gene of interest is inserted at the BamHI site in pBR322, the recombinant plasmid will ...
which was supported by co-culture with OP9 stromal cells (Fig.1a)15. Among the 21 homozygous mutant ESC clones tested, we observed 1 mutant clone that showed a severe disruption in haematopoiesis in OP9 stromal cells (Fig.1b). In this clone, the gene-trap vector was inserted between exo...
This is particularly true for genes involved in the haematopoietic system in mammals, in which diverse genes play delicate and coordinated roles in a time-dependent manner. Mutations in development-related genes are associated with the occurrence of haematopoietic neoplasms in adults. For example, a ...
For example, many scholars have hypothesized that Tutankhamun's death was attributable to an accident, such as a fall from his chariot or a kick by a horse or other animal; septicemia or fat embolism secondary to a femur fracture; murder by a blow to the back of the head; or poisoning....
The gene with the greatest upregulation after in vitro culture was cloned from the genome of B. gibsoni (Wuhan isolate) and characterized by western blotting and indirect immunofluorescence assay for detecting the native form and cellular localization. Results Through laboratory cultivation, multiple ...
Recently there was a major discovery in the scientific research—the mapping of all DNA in a human gene(基因)is complete. Couple of years ago, this seems an impossible task for scientist to accomplish. All this progress in science leads us to believe that the day, when the human being wil...
we could not map any gene residing on accessory chromosomes in this multi-host GWAS (Fig.2and Supplementary Data1). Only eleven genes were shared among PLACP and PLACN (Fig.2and Supplementary Data1), which was expected, since necrosis coverage and pycnidia production levels were highly variab...