Answer to: Draw the expected products in the following reaction sequence: (Image) By signing up, you'll get thousands of step-by-step solutions to...
To solve the given reaction sequence, let's break it down step by step.Step 1: Identify the Reactants The starting compound is acetone (CH₃COCH₃), and it reacts with ethanol (C₂H₅OH
Although a whole-genome sample may be used in sequencing, the lack of a reference sequence necessitates assembling overlapping short sequencing reads into longer contiguous sequences (contigs) (Figure 4A) using computational tools. ...
Transcription rates are regulated by the interactions between RNA polymerase, sigma factor, and promoter DNA sequences in bacteria. However, it remains unclear how non-canonical sequence motifs collectively control transcription rates. Here, we combine massively parallel assays, biophysics, and machine le...
According to this criterion, two mice in the TIDM group were eliminated because their fasting blood glucose levels were less than 11.1 mmol/L. Then, 11 mice from each of the normal and TIDM groups were treated with 15.0 mg/kg Na2HAsO4·12H2O by intragastric administration following fasting ...
The GAS sip* mutant grown to late-exponential growth phase (A600 = 1.0) was resuspended in the spent medium prepared from the SAL growth and incubated at 37 °C for 1 h. Transcript level analyses was performed by quantitative reverse transcription polymerase chain reaction (qRT–PCR)...
To create a stable Flp-In cell line expressing your gene of interest at a site-specific genomic locus, you will perform the following steps: Transfect the Flp-In target site vector, pFRT/lacZeo, into the mammalian cell...
Immunofluorescence staining, real-time polymerase chain reaction, western blotting, and transmission electron microscopy were performed to elucidate the underlying mechanisms. Results BYHWD intervention in ICH reduced neurological deficits. Network pharmacology analysis identified 203 potential therapeutic targets ...
1a, d), consistent with an indirect interaction via VEX1; indeed, the SL-array was strongly enriched following VEX1 ChIP-Seq (Fig. 1d), as determined previously13. We also mapped the VEX2 ChIP-Seq data to a second genome assembly6 that includes hemizygous sub-telomeric sequences (...
The following reaction mixture was incubated at 50 °C for 60 min, and heat inactivated at 70 °C for 15 min: 3 μL of RNase-free water, 2 μL of total RNA, 2 μL of 5× first-strand buffer, 1 μL of 100 μM reverse primers (N rev or C rev), 0.5...