In the following reaction sequence, products (A) to (G) are formed. Product (B) is: View Solution Doubtnut is No.1 Study App and Learning App with Instant Video Solutions for NCERT Class 6, Class 7, Class 8, Class 9, Class 10, Class 11 and Class 12, IIT JEE prep, NEET preparatio...
What are X and Y in the following reaction sequence ? C2H5OH[O]−−→XCl2−−→Y View Solution Identify A,B,C in the following reaction: CH3COCH3H2/Ni−−−→ASOCl2−−−→BKOH/alc.−−−−−−→C
zygotes, and 8-cell embryos using published RNA-seq data (GSE101571)28. As illustrated in Fig.1a, ZGA occurs at the 4–8-cell stage in the human embryo. Maternal mRNAs with reliable sequence annotations and with fragments per kilo
For the following reaction, identify the atoms in the starting material that change hybridization, and indicate the change. Consider the reaction BF3 + NH3 -> F3B - NH3 Describe the changes in hybridization (if any) of the B and N at...
We identified approximately 500 human TFs that encode short periodic blocks of aromatic residues in their intrinsically disordered regions, resembling imperfect prion-like sequences. Mutation of periodic aromatic residues reduced transcriptional activity, whereas increasing the aromatic dispersion of multiple ...
About 100 ng of reverse transcribed total RNA purified from fly heads, was added to the following reaction mix: 1X PCR Buffer, primers (0.2 μM), MgCl2 (1.5 mM), dNTPs (0.2 mM each), and 2U/rxn of Platinum™ Taq DNA Polymerase (Invitrogen). The thermal profile was: 94°C for ...
However, the β-glucosidase responsible for this reaction and its role in plant chemical defence is largely unknown. Herein, we provide evidence demonstrating the existence of a two-component chemical defence system in P. notoginseng. PnGH1, a β-glucosidase localized to the chloroplast, was ...
sequences of the Alu SINE repeat element, indicating the nuclear-retention of RNAs mediated by this motif may be part of a pathway to regulate transcripts that contain Alu insertions (Lubelsky and Ulitsky2018). Some TE insertions, however, may have limited or no impact on the function of a...
The final reaction volume of 20 μL contained 1 μL cDNA, 10 μL of 1 × SYBR Premix Ex Taq, 1 μL of each primer (10 μM), and 7 μL of ddH2O. The reactions were performed under the following conditions: 95 °C, 4 min; 95 °C, 20 s; 60 °C, 30 s (40 cycles). A...
The following criteria were applied to guide the development. (1) Each nucleotide reported in the consensus sequence should derive from at least two independent sequencing reactions using different primers (double strand coverage) to avoid the reporting of phantom mutations and other ambiguous base-...