Plants are complex multi-cellular organisms. Each tissue has its unique role and a variety of cell types that contribute to overall function. Single-cell RNA sequencing (scRNA-seq) has revolutionized our ability to study this cellular diversity. This technology allows us to identify rare cell type...
This included the recommended RNase incubation step to reduce RNA contamination in the final product. The DNA concentration and purity of each sample was assessed by spectrophotometry (Nanodrop, Thermo Scientific, UK), while its integrity was assessed by 0.7% agarose gel electro- phoresis. Each ...
The distribution of CDV antigen and RNA in brain regions was investigated by immunohistochemistry and in situ hybridization (ISH) (Fig.2a–d). For each brain region, the number of animals, which showed CDV+cells, was assessed. The regions where most animals were found to have CDV+cells were...
MN (associated with the University of Minnesota in Duluth) and presented a seminar on the graphical representation of DNA. At that time I too was visiting Basak and attending the seminar where Nandy also presented the DNA plot of the complete human β-globin gene, illustrated...
(YPS163 and YPS606), andCTT1dispensability for both NaCl and ethanol (M22 and YJM308). RNA-seq was performed on these strains comparing an unstressed control to cells treated with either 5% v/v ethanol for 30 min or 0.4 M NaCl for 45 min (timepoints which encompass the peak response ...
By the use of this system, it is feasible to evaluate the requirement for 3D microfluidic culture in MDOTS and the sensitivity towards immune-checkpoint agents of PDOTS, Using RNA sequencing to extrapolate changes in the TME tumor-immune interactions were assessed. Although the results from this ...
(YPS163 and YPS606), andCTT1dispensability for both NaCl and ethanol (M22 and YJM308). RNA-seq was performed on these strains comparing an unstressed control to cells treated with either 5% v/v ethanol for 30 min or 0.4 M NaCl for 45 min (timepoints which encompass the peak response ...
Genetics and epigenetics are tightly linked heritable information classes. Question arises if epigenetics provides just a set of environment dependent instructions, or whether it is integral part of an inheritance system. We argued that in the latter cas
As such, for these and for other modifications, it is often difficult to differentiate between biological and artificially derived modifications. However, sample handling derived PTMs are expected to occur independently from biological cues. Hence, modifications that correlate with the illumination ...
This ability stems from their structure, which comprises a protein capsid containing DNA or RNA fragments. In December 2019, a contagious viral infection broke out in Wuhan, Hubei province, China [1]. The pathogen responsible has been identified as severe acute respiratory syndrome coronavirus 2 (...