Proximity Ligation Assays for In Situ Detection of Innate Immune Activation: Focus on InVitro-Transcribed mRNAinnate immune responsesin vitro-transcribed mRNAprotein-protein interactionsproximity ligation as
An in situ proximity ligation assay (PLA) was performed to detect protein–protein interactions using a Duolink in situ fluorescence kit according to the manufacturer’s protocol (Olink Bioscience, Uppsala, Sweden). In brief, cells were washed with PBS (floating cells were washed away), then fi...
in situ proximity ligation assay (PLA)protein-protein interaction (PPI)signaling pathwayUnderstanding signaling pathway networks via protein-protein interactions (PPIs) at the cellular level is a significant task that has not yet been completed. Here, a systems approach that computationally infers ...
In situ proximity ligation assay (PLA) is a novel, revolutionary technique that can be employed to visualize protein complexes in fixed cells and tissues. This approach enables demonstration of close...doi:10.1007/978-1-4939-6463-5_11Dorota Maszczak-Seneczko...
Duolink®In Situ PLA®探针抗小鼠PLUS 产品介绍: 产品说明 应用 Duolink®邻近连接检测(PLA®) 可在在单分子水平上对固定细胞和组织样品中的蛋白相互作用、翻译后修饰和蛋白表达水平进行内源性检测。 该产品可应用于Duolink®原位荧光方案和Duolink®原位明场方案 ,这取决于所使用的检测试剂。
Proximity ligation assayThe in situ proximity ligation assay (PLA) is capable of detecting single protein events such as protein protein–interactions and posttranslational modifications (e.g., protein phosphorylation) in...doi:10.1007/978-1-0716-0962-0_7Perrino, Brian A....
Duolink®In Situ PLA®Probe Anti-Rabbit PLUS 基本信息 NACRES NA.32 Application【应用】 Duolink®邻位连接技术(PLA®) 能够在单分子水平下在固定细胞和组织样品中对蛋白质相互作用、翻译后修饰和蛋白质表达水平进行内源性检测。 该产品适用于 Duolink®原位荧光检测方案 和 Duolink®原位明场检测方案...
RNAWhole-MountInsituHybridisation ProximityLigationAssay(rISH-PLA),anAssay forDetectingRNA-ProteinComplexesin IntactCells IoannisM.Roussis,MatthewGuille,FionaA.Myers*,GarryP.Scarlett* BiophysicsLaboratories,InstituteofBiomedicalandBiomolecularSciences,UniversityofPortsmouth, Portsmouth,PO12DT,UnitedKingdom *fiona....
To further explore a potential interaction between aggregated α-synuclein and ATP synthase, in situ proximity ligation assay (PLA; Fig.3d) was utilised. 500 nM monomers, oligomers (500 nM monomers, ~5 nM oligomers), or buffer were applied to rat co-cultures and a PLA signal between...
Duolink®In Situ Red Starter Kit Mouse/Rabbit 基本信息 NACRES NA.32 Application【应用】 Duolink®近位连接检测(PLA®)可在固定细胞和组织样本中实现在单分子水平对蛋白互作、翻译后修饰及蛋白表达水平的内源性检测。 根据该Duolink®原位荧光实验方法来使用该产品。还提供了一组简短介绍。