RNA in situ hybridization can be time-consuming and difficult to troubleshoot. Here, we provide an optimized protocol for maize leaf tissue, though it can be applied to other plant tissues such as shoot apical meristems, embryos, and floral organs. We generate three >100 bp unique antisense ...
We present an in situ hybridization protocol to map miRNA subcellular expression in single cells in vivo in four days. Using this protocol, we mapped distinct miRNAs that accumulate in the cytoplasm of one sibling oocyte but not another, dependent on the oocyte developmental stage. Thus, this ...
The in situ hybridization protocol reported here has allowed to complete the previous studies; in fact scarcely expressed transcripts detectable by Northern blot analysis (in our case M3 and M4 mRNAs) [13] can be revealed by in situ hybridization with a combination of two oligonucleotides labelled...
Lecuyer E, Parthasarathy N, Krause HM (2008) Fluorescent in situ hybridization protocols in Drosophila embryos and tissues. Methods Mol Biol 420:289-302Le´cuyer E, Parthasarathy N, Krause HM (2008) Fluorescent in situ hybridization protocols in Drosophila embryos and tissues. Methods Mol Biol ...
Amann and Fuchs provide an update on recent methodological improvements to fluorescencein situhybridization protocols, with a particular focus on whether the original group-specific probes, which were mostly developed more than 10 years ago, are still va
The freshwater planarian Schmidtea mediterranea has emerged as a powerful model for studies of regenerative, stem cell, and germ cell biology. Whole-mount in situ hybridization (WISH) and whole-mount fluorescent in situ hybridization (FISH) are critical
We describe a flow-cytometry-based protocol for intracellular mRNA measurements in nonadherent mammalian cells using fluorescencein situhybridization (FISH) probes. The method, which we call FISH-Flow, allows for high-throughput multiparametric measurements of gene expression, a task that was not feasib...
7) The slides are now ready to enter the CISH staining protocol. Proceed to the denaturation and hybridization steps. You should then add the probe, hybridize overnight, and perform the detection as usual. Need...
7) The slides are now ready to enter the CISH staining protocol. Proceed to the denaturation and hybridization steps. You should then add the probe, hybridize overnight, and perform the detection as usual. Need...
7) The slides are now ready to enter the CISH staining protocol. Proceed to the denaturation and hybridization steps. You should then add the probe, hybridize overnight, and perform the detection as usual. Need more information? Contact us › For Research Use Only. Not for use ...