Deleage C, Chan CN, Busman-Sahay K, Estes JD. Next-generation in situ hybridization approaches to define and quantify HIV and SIV reservoirs in tissue microenvironments. Retrovirology. 2018;15:4.Deleage C, Chan CN, Busman-Sahay K, Estes JD. Next-generation in situ hybridization ...
In situ hybridization and pulsed-field gel analysis define two major minisatellite loci: 1q23 for minisatellite 33.6 and 7q35–q36 for minisatellite 33.15The two classical minisatellite probes, 33.6 and 33.15, were used for in situ hybridization to human metaphase chromosomes. Surprisingly, a single...
Yes, they are ‘in situ’ and ‘ex situ’. The gist is that to define in situ, you need to lay emphasis on the exactness of the intervention or step in terms of its natural or original place, shape, character, size, or position. Let’s move ahead and learn what is in situ ...
Lauter G, Söll I, Hauptmann G: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain. Neural Dev. 2011, 6: 10-10. 10.1186/1749-8104-6-10. Article PubMed Central CAS PubMed Google Scholar Liu G, Amin S, Okuhama...
This chapter presents a comparison of different strategies that may be taken to obtain the desired result with in situ hybridization and immunohistochemistry. It presents variations in the use of in situ hybridization. Depending on the purpose of the individual study, many different protocols and appr...
In situ hybridization In-situ hybridizations were carried out as described in ref.52. Briefly, samples were fixed in PFA on ice, then transferred to a Leica Asp200 for automated tissue infiltration into Leica Histowax. Samples were sectioned at 8 µm and transferred to microscopy slides....
We then validated the expression of both lncRNAs in HeLa cells using a variety of techniques. cDNA generated from polyadenylated RNA was used for rapid amplification of cDNA ends (RACE) to define the locations of the 5′ cap and 3′ end identifying several isoforms forlinc00899andC1QTNF1-AS...
Two proto-oncogenes, Abl and Mos, have been physically localized on their respective mouse chromosomes by in situ hybridization. By means of Robertsonian translocations to facilitate chromosome identification, Abl was regionally assigned to 2B while Mos was assigned to 4A1-A2. Polymorphisms for these...
and even achieving resolutions down to single cells or subcellular levels. The modern age technologies are originated from historical tools that enable simultaneous quantification of gene expression while retaining spatial information. The initial concept of in situ hybridization (ISH) dates back to 1969...
6b, whole-mount in situ hybridization of a Dkk4 probe to fetal skin of Ti+/+ and Ti+/A18V individuals yields patterns that are remarkably similar to the adult Savannah and servaline color markings, respectively. Taken together, these results confirm that the effect of Ticked is not to mask...