As others and we have shown, RNA-binding sites in proteins can readily be determined by UV-induced cross-linking at 254 nm in combination with mass spectrometry (MS)8,9,10. Similar workflows for DNA–protein cross-linking are not yet available, though it is well established that DNA–pro...
The secondary structure of an RNA is formed by interactions between specific nonadjacent bases in the primary nucleotide sequence. The four canonical RNA bases, adenine (A), uracil (U), guanine (G), and cytosine (C), are paired through hydrogen bonds according to the Watson-Crick principle (...
3 demonstrated that large-scale conformational changes of RNA are required for RNA catalysis. And, previously, it was shown that the two ribose-hydroxyls of guanosine as well as N(1)H and NH 2 (2) of guanine base have the potential to form two hydrogen bonds with a phosphate monoanion ...
Currently, m7G modification in tRNA is the most studied. The N7 guanine atom at position 46 of the variable loop is typically methylated by tRNA methyltransferases to generate m7G46 [5]. It interacts with the hydrogen bond of the C13-G22 base pair in the L-type tRNA structure to form a...
Somatic mutations resulting from activating mutations in the gene (GNAS1) encoding the α-subunit of the guanine nucleotide-binding protein (Gsα) during early embryonic life are the main cause of the disease. Because of the mosaic distribution of mutations, it results in a low rate of ...
m7G, a methylation of guanine N7, adds a positive charge to the N atom, affecting the structure of RNA through electrostatic and spatial effects. m7G-cap is important for mRNA shearing and processing, nucleoplasmic translocation and protein synthesis [22]. Abnormal m7G levels are closely associat...
double-stranded DNA, and is held with Hoogsteen or reverse-Hoogsteen bonds. The other strand remains single, and the result is a triple helical structure (Fig.2f). Intermolecular triplexes can occur between DNA molecules, RNA molecules, or as a hybrid involving a DNA and a RNA molecule [41...
95 The susceptibility of a genomic locus to form R-loops is determined by the negative supercoiling generated behind the RNA polymerase and by the DNA strand asymmetry in the cytosine and guanine content, a characteristic known as GC-skewing. During transcription of GC skews, the G-rich RNA ...
pylori infection promoted guanine nucleotide-binding protein subunits β-44 (GNB4) demethylation by activating NF-κB to upregulate TET1, inducing the carcinogenic pathway.161 Besides, F. nucleatum upregulates the transcription of long non-coding RNA (lncRNA) enolase1-intronic transcript 1 (ENO1-IT...
In recent years, with the gradual deepening understanding of m6A and m5C, research on m7G has gradually increased, making m7G modification the next research hotspot in RNA modification. m7G is a modification of RNA guanine (G) by adding methyl to its seventh N under the action of methyltransfer...