成立于1948年的In-N-Out, 虽然已经过了大半个世纪,但是menu依然没有什么变化,仍然是三种汉堡和三种套餐。三种汉堡风别是:双肉饼双芝士汉堡(DoubleDouble);芝士汉堡(Cheese Burger);汉堡(Hamburger)。 不过IN N OUT是有秘密菜单(Secret Menu),大家以后可以照着...
In-N-Out 原本还有一道特色是顾客可以指定一个汉堡中放置任意数量的肉饼与起士(加价需顾客自付),但自2004年一位顾客要求单只汉堡中放置100片肉饼与100片起士后这家汉堡店还是决定禁止四片肉饼与四片起士以上的汉堡。 现在来回答已经吃过In-N-Out的朋友,其实 In-N-Out 还有很多隐藏菜单,这大多数是非常了解 In-...
Recent mechanistic models from radiobiology have emerged, pointing out that the radiation-induced nucleo-shuttling of the ATM protein (RIANS) initiates the recognition and the repair of DNA double-strand breaks (DSB) and the final response to genotoxic stress. In order to document the role of ...
a, Discovery analyses were conducted in UK Biobank (n= 419,668) and replication was conducted in individuals from the MCPS and PGR study (n= 167,359). The means of the effect size estimates are presented with 95% CIs and were converted to kg m−2. Extended data can be found in...
Large double-stranded DNA (dsDNA) viruses have the most protein clusters per species and, despite constituting only 14 of the 132 viral families in the dataset, account for the majority of viral proteins (Fig. 1d,f). As expected, protein cluster count correlates strongly with genome size (...
(GeneVec) for gene sequences, this representation can be widely used in applications of deep learning in proteomics and genomics. Biovectors are basically n-gram character skip-gram wordvectors for biological sequences (DNA, RNA, and Protein). In this work, we have explored biophysical and ...
The N-terminus of the PnGH1 protein contains a chloroplast transit peptide, which is consistent with the identified subcellular localization of PnGH1 in the chloroplast. Upon cellular disruption caused by an event such as a pathogen attack, toxic defence compounds are bioactivated by removing a ...
Pubi: maize ubiquitin1 promoter; Pd35S: doubled enhanced CaMV 35S promoter; Pactin, rice actin promoter; Tnos: nopaline synthase gene terminator; T35S: CaMV 35S gene terminator; gfp: green fluorescent protein coding region; gusi: β-glucuronidase (gusA) protein coding region; hpt: ...
Double mutant ΔczrAΔnczA was obtained by introducing the pNPTS138 vector containing the 5′ and 3′-flanking regions of czrA into the ΔnczA strain. PCR products using primers RND15/RND16 (3243 bp) and RND17/RND18 (3132 bp), containing the coding regions of czc1 and czc2 ...
Protein–DNA interactions are key to the functionality and stability of the genome. Identification and mapping of protein–DNA interaction interfaces and sites is crucial for understanding DNA-dependent processes. Here, we present a workflow that allows mass spectrometric (MS) identification of proteins...