Extraction of films with methanol and characterization of the extracts by gel permeation chromatography revealed the presence of low-molecular-weight polymers. Results have shown that trypsin has the ability to induce degradation in PEU, the soft segment being most affected, particularly the CH2-O ...
Melting curves were systematically run at the completion of each assay to ensure the presence of a single amplification product and absence of primer-dimers or unspecific qPCR products. Relative quantification of the selected transcripts was assessed by the ΔΔCt method using the 16S ribosomal RNA ...