Hsp60 shares sequence and structural similarities with orthologs from different species and, thus, in extracellular locations, it cross-reacts with antibodies and immune cells activated by foreign immunogens. Hsp60 can also stimulate production of proinflammatory cytokines. Hsp60 can induce innate TLR4...
118) backbone, in which the chloramphenicol-resistance cassette was replaced with DHFR, in frame with a Ty tag between the SAG1 promoter and 3′-UTR. Parasites were selected using pyrimethamine, cloned by limiting dilution and positive clones selected by immunofluorescence microscopy for the Ty tag...
The involvement of Heat Shock Proteins (HSP) in cancer development and progression is a widely debated topic. The objective of the present study was to evaluate the presence and expression of HSP60 and HSP10 in a series of large bowel carcinomas and locoregional lymph nodes with and without ...
As an isoform of PTEN, PTENα is initially translated from a CUG codon upstream of and in-frame with the coding region of canonical PTEN15. As shown in Supplementary Fig. 1b, phosphatase-inactive mutations impaired the phosphatase activity of PTEN and its isoform PTENα. To assess the effect...
We confirmed that HSP60 knockdown increased cell proliferation, whereas its overexpression decreased cell growth. Proteomics and metabolomics revealed that HSP60 knockdown promoted Warburg-like phenotypes with enhanced glycolysis and decreased mitochondrial activity. Consistent with this finding, isotope tracing...
Neonatal Death and Heart Failure in Mouse with Transgenic HSP60 Expression Tsung-Hsien Chen, 1 Shan-Wen Liu, 1,2 Mei-Ru Chen, 1 Kuan-Hung Cho, 1 Tzu-Yin Chen, 1 Pao-Hsien Chu, 3 Yu-Ying Kao, 4 Ching-Han Hsu, 2 and Kurt Ming-Chao Lin 1 1 Institute o Biomedical Engineering and...
Look great in every frame The Samsung Galaxy J7 lets you take bright and vivid selfies every time with more accurate detail even in low light thanks to its front LED and selfie flashes. You can also control the shutter by simple hand gesture. ...
摘要!目的采用PCR法进行嗜肺军团菌lugA和Hsp60二基因的拼接融合a克隆并检测该融合基因在原核系统中表达情况a为进一步研究其免疫性能作必要的准备O方法采用重组聚合酶链反应a设计引物首先分别从嗜肺军团菌基因组DNA中扩得待融合端互补的军团菌毒力基因(lugA)和热休克蛋白60基因(Hsp60)a经琼脂糖电泳纯化回收a再以适量...
MMGL isoform 4 interacts with the C1-C2 region of cMyBPC. A. Representative image of live cell fluorescence microscopy showing co-localization of cMyBPC and MMGL isoform 4. Each panel represents a single frame of the 25 images that were captured for the vertical Z-stack. The first four pa...
To correct for assembly errors associated with Nanopore sequencing, Proovframe v0.9.7 (and diamond v2.0.8) (https://github.com/thackl/proovframe), was used to correct indels by aligning polished BFG genomes to Genbank nr database (release 245), and replacing indel regions with Ns to im...