Fixing Mistakes in DNA Replication DNA polymeraseenzymes are amazingly particular with respect to their choice of nucleotides during DNA synthesis, ensuring that the bases added to a growing strand are correctly paired with their complements on the template strand (i.e., A's with T's, and C'...
Specialized DNA helicases efficiently unwind G-quadruplex (G4) DNA and other forms of alternative DNA structures such as telomeric displacement loops (T-loops). Emerging evidence suggests that DNA helicases that unwind non-conventional DNA structures have important roles in the replication or repair ...
The potential for Cas9-sgRNA binding and cleavage of sequences that are highly similar to the target DNA sequence is another hindrance to clinical translation of CRISPR/Cas9-edited CAR T cells. This could result in mutations at undesirable locations in the DNA [60]. Thus CRISPR/Cas9 is in ...
Answer true or false: When nucleotides form base pairs between strands, A pairs with G (with two bonds), and T pairs with C (with three bonds). True or False: Each mRNA molecule is used to produce one copy of a polypeptide molecule. Is ...
PEA uses pairs of antibodies equipped with DNA reporter molecules, producing DNA amplicons, which are subsequently quantified using a Fluidigm BioMark HD real-time polymerase chain reaction platform. The method has acceptable reproducibility and repeatability, with mean intra-assay and interassay ...
(4,286 bp total) ofSaccharina japonicamorphological forms.The topology of trees obtained with Maximum likelihood and Neighbor-joining methods were congruent. Numbers at the nodes are bootstrap percent support values based on 1,000 replications in Maximum likelihood analysis.Saccharina latissimawas ...
Genomic DNA was prepared using TIANamp Genomic DNA Kit (DP304, TIANGEN, Beijing China) following the manufacturer’s instructions. At least 10 μg genomic DNA of each sample was used to construct paired-end sequencing libraries, with the insert size of 300–400 base pairs according to Illumina...
DNA replication-dependent intranuclear relocation of double minute chromatin J. Cell Sci., 111 (Pt 22) (1998), pp. 3275-3285 Google Scholar [60] F.W. Alt, R.E. Kellems, J.R. Bertino, R.T. Schimke Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants...
slipped strand mispairing is a mutation process that occurs during DNA replication, which is one explanation for the origin and evolution of repetitive DNA sequences126. TRs, especially STRs, are extremely unstable in terms of length, sequence composition, and copy number, with mutation rates typi...
The quickly accumulating ribosome profiling data is an insightful resource for studying the critical details of translation regulation under various biological contexts. Rocaglamide A (RocA), an antitumor heterotricyclic natural compound, has been shown