CF 0F 1, H +-translocating ATPase (‘ATP-synthase’) from chloroplastsME 1, isolated hydrophilic part of the H + translocating ATPase from mitochondriaChl, chlorophyllATP-hydrolysis was measured with thylakoids under uni-site conditions. The rate constant for the ATP binding is 10 6 M 1 ·...
Single-turnover flash-induced ATP synthesis in chloroplasts was measured in situ with the luciferin luminescence method. In dark-adapted chloroplasts the first flashes only induce ATP hydrolysis. Once the reversible ATPase is fully activated, ATP hydrolysis persists for extended periods of darkness and...
Functional Studies on the Reaction Pattern of the H+-ATP-Synthase in Spinach Chloroplasts Strelow F, Tran-Anh T and Rumberg B (1990) Functional studies on the reaction pattern of the H + -ATP synthase in spinach chloroplasts. In: Baltscheffsky M (ed) Current Research in Photosynthesis, ...
In respiration and photosynthesis, electron transport generates a proton motive force across the membrane, which drives FoF1-ATP synthase1,2. In eukaryotes, these reactions are performed in mitochondria and chloroplasts as the remnants of ancient prokaryotic energy production systems3. A series of redo...
Proteins newly formed from labeled amino acids by isolated intact pea chloroplasts are not entirely stable. Between 20 and 35% of the labeled protein is degraded over a 20–30 min incubation period in pulse-chase experiments. Protein degration is prevented when chloroplast ATP level drops, as in...
PBR1 regulatesYcf1translation by binding to its mRNA. (a) PBR1-YFP (yellow fluorescent protein) signals are targeted to chloroplasts as examined in guard cells of epidermal peels. (b) The representative mass spectra for identification of Ycf1 protein in samples extracted from wild-type (WT) and...
Mitochondrial development is also affected by chloroplasts. In rice, a single chloroplast mutation impairs mitochondrial development [49]. Therefore, mitochondrial gene defects might also affect chloroplast development. AT5G08670 encodes an ATP synthase beta-subunit in mitochondria. The mitochondrial beta-...
The energy requirement for protein transport into chloroplast was assayed under conditions that perm it to distinguish whether the posttranslational translocation is dependent on ATP or whether a membrane potential across the chloroplast envelope can dri
Since chloroplasts of both mutants were altered with respect to their size and shape, we examined their effects on the morphology of accumulated starch granules. Reduction of starch granule size, inferred from the cross-section area, was significant inAtidd5(P-value = 1.38E-10), but not...
but absorb them by food uptake from the chloroplasts of plants/algae1,2. The polyenic-conjugated structure of carotenoids (C = C alternated with C-C leading to π delocalised electrons) appears an efficient mode to transport electrons across the lipid bilayer, putatively funnelling them to the ...