3a), s-DR-bound GUVs are introduced in a GFP (∼500 nM) containing solution. At equilibrium, the GUV is spherically shaped and no GFP transport across the membrane is visible (Fig. 3a). Upon the addition of unlocking strands, the expected GUV reshaping emerges, while the interior of...
electrochemical proton gradients. The generation of this gradient has a major role in providing the energy for secondary active transport across the plasma membrane. The plant plasma membrane H+-ATPase is a proton pump which plays a central role in physiological functions such as nutrient uptake, ...
Studies using the H+-ATPase inhibitor ebselen or a yeast genetic strain with reduced H+-ATPase found reduced τi1, notwithstanding high ATP. Steady-state water exchange correlates with H+-ATPase activity. At volume steady state, water is cycling across the plasma membrane in response to ...
3d). As many transporters function as exchangers, mediating both import and export of amino acids10,12, we also determined net transport across the plasma membrane by measuring amino acid consumption from the culture medium (Extended Data Fig. 3d). We determined absolute import and net transport...
(net influx clearance of the probe from plasma or blood to tissue),k2(an efflux rate constant), and the two compartment model’sK1(distributional clearance into the tissue, CL12),k2(the transport process from the tissue back into the blood), andk3andk4(the bidirectional exchange between ...
membrane disruptions and repair mechanisms in both physiological and pathological conditions, and across multiple cell types, albeit to different degrees. Fundamentally, irrespective of the source of membrane disruption, aberrant calcium influx is the common stimulus that activates the membrane repair ...
. The insect V-ATPase, a plasma-membrane proton pump energizing secondary active transport - molecular analysis of electrogenic potassium transport in the ... H Wieczorek - 《Journal of Experimental Biology》 被引量: 196发表: 1992年 THE INSECT V-ATPase, A PLASMA MEMBRANE PROTON PUMP ENERGIZING ...
When MVBs fuse to the plasma membrane, exosomes are released into the extracellular environment. EVs carry active substances such as soluble proteins, lipids, metabolites, DNA, and RNAs (mRNA, miRNA, and other small regulatory RNA). DownLoad: Full-Size Img PowerPoint EVs Derived from Adipose ...
A mechanism of cytotoxicity related to trogocytosis mediated by an active process of plasma membrane transfer between interacting cells. Rights and permissions Reprints and permissions About this article Cite this article Jaillon, S., Ponzetta, A., Di Mitri, D. et al. Neutrophil diversity and pla...
In human and other mammalian cells, transport of L-lactate across plasma membranes is mainly catalyzed by monocarboxylate transporters (MCTs) of the SLC16 solute carrier family. MCTs play an important role in cancer metabolism and are promising targets f