Physical exercise is a cornerstone for preventing diet-induced obesity, while it is unclear whether physical exercise could offset high-fat, high-calories diet (HFCD)-induced cardiac dysfunction. Here, mice were fed with HFCD and simultaneously subjected to physical exercise. As expected, physical ...
Briefly, for incubation with the mRNA, a pumping system was used to mix equal volumes of two aqueous phases, comprising mRNA and liposomes. The mRNA was synthesized at BioNTech using internal protocols. The liposomes consisted of DOTMA and DOPE in a molar ratio of 2:1. Concentrations were ...
The current-control method with the porous plug installed was put to one final experimental test where it was used to measure the electrophoretic mobility of the PNIPAM in a solution containing 200 mM NaSCN. The phase data provided in Fig. 6 show that the methodology presented here can reliabl...
Fig. 2. Study of the influence of NaH2PO4/NaOH buffer solution in the analytical signal, using a solution containing 50 μg/mL KTP and 1.5 μM QDs of 3.6 nm diameter. KTP = ketoprofen; RSD = relative standard deviation. Once the optimum pH was fixed, the optimum diameter and concentratio...
Lysates containing equal concentration of 50 μg proteins were separated by 8%, 12% or 15% (w/v) sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a polyvinylidene difluoride membrane, which was then blocked in 5% nonfat milk at room temperature for 1 h. The ...
Add 350 µl of Wash Buffer I to the spin cartridge. Centrifuge at 12,000 × g for 15 seconds at room temperature. Discard the flow-through, and re-insert the cartridge in the tube. In a separate RNase-free tube, prepare a DN...
Protein samples are simply added to the diluted NanoOrange reagent in a lipid-containing medium, and the mixtures are heated at 95°C for 10 minutes. After cooling the mixtures to room temperature, their fluorescence emissions ...
void example() { struct A { int boo(); // warning C4822: Local class member function doesn't have a body }; } Function template bodies containing if constexpr statementsIn Visual Studio 2019 under /std:c++20 or /std:c++latest, template function bodies that have if constexpr statements ...
while the other side of the substrate-binding pocket exhibited a wide, open entrance (Fig.3d), making it possible for the protein to accommodate various PPD-type ginsenosides with different substituents at C-20. PnGH1 retained the glutamic acid-containing catalytic motifs TFN(E/D)P and (V/Y...
3b,c, showing the current density as a function of the potential of the Pt foil cathode and RuO2–TiO2/Ti dimensionally stable anode (DSA), respectively. These measurements were carried out in 1.5 M NaBr electrolytes containing different borate buffer concentrations (as indicated in the legend...