S1 [19]. Three hundred fifty microliters of cold lysis buffer was added to the brain tissue and sonicated (5 rounds: 3 s—pulse, 12 s—pause, amp 7). Tissue was triturated ten times through a 26-gauge needle (VWR, BD305111) and passed through Qiagen shredder (Qiagen, 79654) ...
Twenty microliters of MTS was then added to each well, and after 3 h at 37 °C, 490 nm absorbance of the formazan product was measured using a microplate reader (Clariostar; BMG Labtech, Cary, NC, USA). The quantity of product measured by this method is directly proportional to...
Ten microliters were injected into the LC-MS/MS system consisting of an Acquity UPLC system coupled to a triple quadrupole (TQS Micro) mass spectrometer. Chromatographic separation was performed in an Acquity BEH C18 column (100 mm × 2.1 mm i.d., 1.7 μm) (Waters Associates) ...
One hundred microliters of genipin solution [2% (w/v)] was serially added to 10 ml aliquots of the CG/PHA/PBT solution, stirred for 10 min until blended, and then poured into plates for gel formation. In the following sections, pure CG hydrogels are denoted as the CG group, ...
Twenty microliters of 50 × DAB stock solution was added to each 1 mL of DAB dilution solution for later use as a chromogen. The slides were counterstained with hematoxylin and rinsed under running water. Image analysis was performed by one skilled evaluator through an optical microscope (...
Twenty microliters of a sample was extracted with 120 μL of cold methanol/acetonitrile (1:1, v/v) and vortexed for 1 min. The mixture was homogenized for 5 min and subsequently centrifuged at 14,000×g for 15 min at 4 °C. In addition, pooled QC samples were also prepared by ...
Splitless injection of up to hundreds of microliters of liquid samples in capillary GC: Part 1, concept Grob, K., Brem, S. and Froehlich, D., Splitless injection of up to hundreds of microliters of liquid samples in capillary GC: Part 1, Concept. J... K Grob,S Brem,D Frhlich - ...
A modified micro solid-phase radioimmunoassay () for antibody to (anti-HAV) was developed. This double antibody procedure was performed by coating the surface of a polyvinyl microtiter plate "well" with 200 microliter of a 1:1,000 diluti... DANIELW. BRADLEY,HOWARD A. FIELDS,KAREN A. McC...
Six microliters of aggregated wild-type and mutant KRT81–90 samples (taken from in vitro aggregation experiments) was spotted onto Formvar Carbon film 400 mesh copper grids (Electron Microscopy Sciences) and incubated for 4 minutes. Grids were stained with 6 µL uranyl acetate solution (2%...
PtenandMtornormalised to18S, in control and F2 ovaries; **P < 0.01, ***P < 0.005 (h). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin (i) show that the AKT pathway is activated in F2 ovaries (n = 3), as indicated by the increased P-...