性状Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4 溶解方法Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/ml in sterile distilled water by gentle pipetting 2-3 times, don’t vortex 存储-20°C for 12 mont...
Kenilworth, NJ, USA) and lyophilized using a vacuum concentrator. The protein pellet was dissolved in 8 M urea/20 mM methylamine solution and protein concentration was estimated using QubitTMProtein Assay Kit (Thermo Fisher Scientific). For secretome samples, 10 µg...
2a, b, g, h). Oral intake of ERGO also suppressed the increments in age-related plasma BMs such as creatinine, SDMA, urea, ADMA, quinolinic acid, and kynurenine (Fig. 4a–d, f, g), and ERGO enhanced object recognition memory and improved learning and memory ability in aged mice (...
Blend membranes of chitin/cellulose from 12 : 50 to 12 : 250 were successfully prepared from cotton linters in 1.5 M NaOH/0.65 M thirourea solution system... L Zhang,G Ji,Y Du - 《Journal of Applied Polymer Science》 被引量: 36发表: 2010年 Structure and properties of regenerated cellul...
Guanosine 3′,5′-bis(pyrophosphate) (ppGpp) functions as a second messenger in bacteria to adjust their physiology in response to environmental changes. In recent years, the ppGpp-specific hydrolase, metazoan SpoT homolog-1 (Mesh1), was shown to have im
SRM 925 VMA (Clinical) 1 gSRM 927e Bovine Serum Albumin (7%, solution)牛血清白蛋白(7%,溶液) 10 x 2.2mLSRM 928 Lead Nitrate (Clinical)*(临床) 30gSRM 929a Magnesium Gluconate葡萄糖酸镁 5gSRM 931h Liquid Absorbance Standard for Ultraviolet and Visible Spectrophotometry set(12)SRM 935a ...
A new, sizable family of 2D transition metal carbonitrides, carbides, and nitrides known as MXenes has attracted a lot of attention in recent years. This i
Compared with control rats, AAN rats showed anemia, increased serum urea and creatinine, progressive renal interstitial fibrosis, activation of nuclear factor-kappa B, and up-regulation of pro-inflammatory, pro-oxidant, and pro-fibrotic proteins at weeks 8 and 12. However, no significant difference...
A high flux 1.4 m2 polyethersulfone dialyzer was used (Bellco Diapes 140G, Genpharm, Etobicoke, Ontario, Canada). The final concentration of solutes in the dialysate solution was sodium 140, bicarbonate 35, potassium 3.0, calcium 1.5, and magnesium 0.75 mmol/l. If hypophosphatemia developed ...
(IAA, Sigma, #SLCD4031). The solution was diluted with 70 µL 100 mM ABB and then a 2-step overnight tryptic digestion (Hualishi Tech. Ltd, Beijing, China), at an enzyme/substrate ratio of 1:60 for 4 h and 12 h, successively. Thereafter, the digestion was quenched ...