An instructional card for a first aid kit that gives instructions for administering first aid by audio means, visual means, written means, graphical means or a combination of means thereof. The instructional card may be identified as corresponding to a pack of first aid supplies to be used to...
Capped RNA for injection was transcribed using the mMessage mMachine Sp6 or T7 kit (ThermoFisher Scientific) followed by LiCl precipitation. For live imaging experiments, zebrafish embryos were injected with 25 pg H2B-mScarlet-I and 400 pg of modErk-KTR-Clover, modErk-KTRAAA-Clover or modErk...
We addressed the bottlenecks of Nextera library construction to produce a modified protocol which harnesses the full power of the Nextera kit and allows the reproducible construction of libraries on a high-throughput scale reducing the associated cost of the kit.Back...
The pBBK was linearized using XbaI digestion, and PhiC31o mRNA was then in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Kit (Ambion, Austin, TX) followed by purification of mRNA using MEGAclear Kit (Ambion). To avoid clogging during microinjection, mRNAs were filtered by passing ...
(b) Partial ratio plot for the mouse chromosome 6 region encompassing the ROSA26 amplicon in tumor 9 (right) and copy number of the MYCN transgene in 13 tumors from heterozygous LSL-MYCN;Dbh-iCre mice (+/ − ), as assessed by qPCR (insert left). Bars represent mice with normal ...
Injury to the heart results in cardiomyocyte cell death and can lead to pathological remodeling of remaining cells, contributing to heart failure. Despite the therapeutic potential of new drugs and small molecules, there remains a gap in the ability to e
Transformants were cultured overnight, and plasmids were extracted using a Qiaprep Spin Miniprep Kit (Qiagen). Plasmids were confirmed via both a restriction enzyme digest check and Sanger sequencing. Cloning Yeast Diversifying Base Editor (yDBE) Constructs The amino acid sequence for MCP-AID*Δ34...
First, a WGS library with an average insert size of 3.5 kb was constructed. End sequencing of 489,216 clones was then performed using the ABI 3730xl DNA Analyzers (Applied Biosystems). After quality and vector clipping, the WGS and fosmid end sequence data were assembled by the PCAP.REP...
First, a WGS library with an average insert size of 3.5 kb was constructed. End sequencing of 489,216 clones was then performed using the ABI 3730xl DNA Analyzers (Applied Biosystems). After quality and vector clipping, the WGS and fosmid end sequence data were assembled by the PCAP.REP ...
The genome sequence ofR. varieornatuswas determined by using a combination of the Sanger and Illumina technologies. First, a WGS library with an average insert size of 3.5 kb was constructed. End sequencing of 489,216 clones was then performed using the ABI 3730xl DNA Analyzers (Applied Bio...