We hypothesize that CNAs are a major determinant of allelic methylation at imprinted DMRs. To ensure that the profiles obtained using the COSMIC HM450k methylation data set accurately reflected the methylation pattern at imprinted DMRs, we compared the profiles for five cell lines with those obtained...
Animals were housed at a density of 3–4 per cage in a temperature-controlled room (20–22°C) with a 12 hr light/dark cycle, except for thermoneutrality experiments, when cages were placed on a hot pad at 30°C. Cold-challenged animals were removed from the nest and separated at ...
2.5. Real-Time PCR cDNA synthesis was performed with 1 µg total RNA using PrimeScript™ RT Master Mix (Perfect Real Time) and PrimeScript™ RT reagent Kit (Perfect Real Time) according to the manufacturer's instructions (TaKaRa, Dalian, China). All the primers were synthesized by ...