The AT-rich genes are prone to be tissue-specific (silenced in most tissues), while the GC-rich genes tend to be housekeeping (expressed in many tissues). This paper reports another important property of DNA base composition, which can affect repertoire of genes with high AT content. The ...
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While data with immunotherapy have been less robust in the microsatellite stable (MSS) population, results of a small phase Ib trial presented at the 2020 Gastrointestinal Cancers Symposium showed that the combination of regorafenib (Stivarga) and nivolumab led to an objective response rate of 29% ...
The propensity of a sequence to form RNA secondary structures can be predicted from high GC content and low minimum free energy (MFE) of predicted secondary RNA structures [2, 29]. We used RNAfold [30] to compute the MFE prediction per 5’UTR. The most LoF intolerant genes had lower ...
This included assembly statistics, genome quality (completeness and contamination estimates) and properties (e.g., GC content, estimated genome size, number of contigs). Number of tRNAs and the corresponding encoded amino acids were predicted using tRNA scan SE-1.23 using the domain-specific ...
PhD1, Michelle Clemens, MS, CGC1 and Aleksandar Rajkovic, MD, PhD1 Background: Noninvasive prenatal screening (NIPS) by next-gen- eration sequencing of cell-free DNA (cfDNA) in maternal plasma is used to screen for common aneuploidies such as trisomy 21 in high risk pregnancies...
Chosen parameters were: product size range 100–150 bp, primer size 20–22 bp, primer Tm 57–60 °C (with maximum Tm difference = 2 °C) and GC content 45–60%. Primer combinations were custom-ordered from a commercial supplier (Integrated DNA Technology, USA) and ...
The N-terminal primase domain of T7 gp4 comprises seven conserved sequence motifs, I, II, III, IV, V, VI, and an RNA polymerase basic domain. The putative primase domain of metazoan mitochondrial DNA helicases has diverged from T7 gp4 and in particular, the primase domain of vertebrates ...
In this study, we “top-down” enriched the lignin-degrading community from the coastal intertidal wetlands of the East China Sea. Through high-throughput sequencing and physiological analyses of the community and isolates, we not only characterized the taxonomic and functional gene profiles of the ...
The features ofThymus mongolicusgenome.aThe landscape ofT. mongolicusgenome. The circus plot from the outer to the inner circle represents chromosome-scale pseudochromosomes (Chr01–Chr12) (I), GC content (II), repeat element (III-1), Ty1/copia retrotransposons (III-2), Ty3/gypsy retrotransp...