Method of isolation of dna The DNA isolated can be catenated closed circular DNA, such as kinetoplast DNA of Trypanosoma cruzi. The purified DNA fragments can be used for amplification in a sequence-based DNA amplification system employing primers that hybridize to... DS Sigman,L Simpson,H Avil...
DNA copying is called DNA replication. In this process one copy each of replicated DNA will be passed on two daughter cells. Each cell formed will have its own cellular apparatus to control the activities of daughter cells. It maintains the body design
However, these tests' performance relies on the isolation of DNA in a good concentration. The standard procedures of commercial DNA extraction kits are usually insufficient for this purpose due to the tough walls of spores. This study aimed to test the significance of pretreatments by glass beads...
This report describes the isolation and preliminary characterization of a 5.0-kilobase-pair (kbp) EcoRI DNA restriction fragment carrying the catBCDE genes from Acinetobacter calcoaceticus. The respective genes encode enzymes that cataly... MS Shanley,EL Neidle,RE Parales,... - 《Journal of Bacter...
Isolation of plasmid DNA from mycobacteria using a resin-based alkaline lysis kit The characterization of recombinant mycobacterial genes has routinely been time-consuming and difficult because of the laborious procedures required for is... CH King,BB Plikaytis,TM Shinnick - 《Biotechniques》 被引量...
Genetic studies of wild animals often employ neutral markers such as mitochondrial d-loop DNA (mtDNA), microsatellites or single nucleotide polymorphism (SNPs) to estimate the amount of variation present in individuals and populations [22–24]. While these markers are very informative for phylogenetic...
An inventory of the bZIP transcription factors in Arabidopsis, which are classified in ten groups, is presented here as a basis for future functional analy... M Jakoby,B Weisshaar,Drge-Laser W,... - 《Trends in Plant Science》 被引量: 2319发表: 2002年 Isolation of the Arabidopsis ABI3...
Genetic studies of wild animals often employ neutral markers such as mitochondrial d-loop DNA (mtDNA), microsatellites or single nucleotide polymorphism (SNPs) to estimate the amount of variation present in individuals and populations [22–24]. While these markers are very informative for phylogenetic...
Moreover, isolation of local strain (isolates) from harvested tachyzoites was conducted after confirmation using PCR. Zoonotic importance was discussed and suggested recommendations were recorded to control such disease of great economic importance in veterinary field as well as its public health ...
To determine the role of the C-terminal region of Cellvibrio gilvus β-glucosidase, a deletion mutant was constructed lacking five amino acid residues (RGRAR), three of which were arginine, from the C-terminal end. The mutant, designated ΔRGRAR, could be folded into an active form when ex...