A flow cytometry method has been introduced into the routine investigation of whole bone marrow samples following red blood cell lysis on the basis of a primary CD45/side scatter (SSC) gating procedure. Blast cells were first identified by CD45/SSC gating in 74 cases of acute myeloid leukemia...
blood has been previously described, the method is of limited utility in traditional systems, which use hydrodynamic forces to orient cells in the flow stream [2]. Moreover, significant sample dilu...
Whole blood lysisDensity gradient separationImmunophenotyping of acute leukemia using flow cytometry after density gradient separation (dg-sep) of mononuclear cells is the international gold standard. But destroying red cells by whole blood lysis (wb-lysis) after direct staining has found a b...
The typical immunophenotyping panel to evaluate is T, B, and NK cells in peripheral blood (Figure 7.3). The cell surface markers evaluated would be CD3+ T lymphocytes, CD3+/CD4+ T-helper lymphocytes, CD3+/CD8+ T-cytotoxic lymphocytes and CD20+ B lymphocytes. The NK cell phenotype is ...
Human whole blood was stained and interrogated using the probes and configurations described in Table 1. (A) A fluorescence threshold was set on Pacific Orange™ fluorescence (CD45), and events coincident with red blood cells were excl...
(BAL) but not in peripheral blood, suggesting unclear inflammatory mechanisms that could influence γδ T-cells counts in the periphery55. Interestingly, γδ T-cells, in particular VD2, are known to participate in influenza immune response56, and actively recruit and activate neutrophils to the...
(BAL) but not in peripheral blood, suggesting unclear inflammatory mechanisms that could influence γδ T-cells counts in the periphery55. Interestingly, γδ T-cells, in particular VD2, are known to participate in influenza immune response56, and actively recruit and activate neutrophils to the...
CD11c staining from BAL cells and blood neutrophils of day 3 LPS-challenged HckF/F mice (blue: BAL cells, red: blood neutrophils; n=3–5). (j–l) Flow cytometry used to calculate total AMΦ, monocyte, neutrophil, and lymphocyte numbers (n=3–5, *P<0.05, **P<0.01, two-tailed ...
- 《Blood》 被引量: 421发表: 1997年 Evaluation of a 12‐color flow cytometry panel to study lymphocyte, monocyte, and dendritic cell subsets in humans Monitoring changes in human immune cell populations such as lymphocytes, monocytes, and dendritic cells (DCs) during infectious diseases like ...
To evaluate functionality of different immune cells, flow cytometry was performed from fresh blood or frozen peripheral mononuclear cells (PBMCs) previously isolated with Histopaque (Sigma-Aldrich, St Louis, Mo). Three flow cytometry panels were performed: (1) NK and T-cell functionality, (2) me...