Importantly, HER2 genetic reflex test for all discordant cases confirmed the scores obtained by the microfluidic technique. Overall, the microfluidic-based IHC represents a clinically validated equivalent approach to the standard chromogenic staining for rapid, accurate, and automated breast cancer diagnosis...
Comprehensive immunohistochemical analysis of Her-2/neu oncoprotein overexpression in breast cancer: HercepTest™ (Dako) for manual testing and Her-2/neu... (4B5) for immunohistochemical (IHC) determination of Her2 status in breast cancer: comparison with CB11, fluorescence in situ hybridization ...
Using this technique, the pathologist can find out where in the body the cancer originated, by looking for cells which don't belong in the sample. For example, breast cells in a liver would indicate that the tumor was actually breast cancer which had metastasized to the liver. The ...
A new standardized immunohistochemistry (IHC) control for breast cancer testing comprises formalin-fixed human epidermal growth factor receptor 2, estrogen receptor, or progesterone receptor peptide antigens covalently attached to 8-m glass beads. The antigen-coated beads are suspended in a liquid matrix...
Fluorescencein situhybridization (FISH) is increasingly being recognized as the most accurate and predictive test for HER2/neugene amplification and response to therapy in breast cancer. In the present study we investigated HER-2/neugene amplification by FISH in breast carcinoma tissue specimens and ...
Overall, there is a lack of uniform guidelines in the evaluation of PD-L1 that account for different IHC assays, different cell types, and different cutoff values across tumor types. While breast cancer is not a robustly immunogenic tumor type overall, certain subtypes, largely the estrogen ...
received: 10 May 2015 accepted: 27 August 2015 Published: 25 September 2015 Molecular portraits revealing the heterogeneity of breast tumor subtypes defined using immunohistochemistry markers Xiaofeng Dai1,3, Yang Li2, Zhonghu Bai1,3 & Xu-Qing Tang2 Breast cancer is highly heterogeneous...
IHC slides were evaluated semiquantitatively based on the Allread scoring system which is clinically used in hormone receptor evaluation of breast cancer20. Staining intensity score (0: negative, 1: weak, 2: moderate, 3: strong) and proportional score based on percentages of stained cells (0: ...
10-Minute IHC for Mohs Surgeons ihcDirect assays use the patented Novodiax polymerized HRP process to directly conjugate onto primary antibodies. This yields fast, simple, and sensitive IHC test results in minutes. Learn more Q-STAIN®X
Clinicopathological character- istics are shown in Table 1 (test cohort). Four tumor biopsies (0.4–0.8 cm3 in size) were removed from each breast tumor (Figure 1). All tissue samples were fixed in 4% buffered formaldehyde for 24–48 h. After fixation, breast cancer tissue collected for ...