Cell Analysis Learning Center Cell Analysis Support Center What is immunohistochemistry? Immunohistochemistry (IHC) is a technique that uses an antibody to bind a specific antigen in a tissue section and is vi
Cell Analysis Learning Center Cell Analysis Support Center What is immunohistochemistry? Immunohistochemistry (IHC) is a technique that uses an antibody to bind a specific antigen in a tissue section and is visualized with a fluorophore or colored substrate. Locations of stained proteins helps us und...
Many antibodies that perform well in other assays do not work well in IHC and can have undesirable effects, including producing weak or no signal, showing nonspecific background staining that interferes with analysis, or even causing false positive signals. Vector Laboratories provides a portfolio of...
There are no conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome. Acknowledgements The authors would like to thank Dr. Victor Yunes Guimarães for advices with the IHC analysis.References...
Image analysis showed that within the CD8+ population, median % TIM-3+/CD8+ TILs were primarily intratumoral (18.0%), with fewer in peritumoral (9.4%) and nontumoral (6.4%) regions (intratumoral vs peritumoral, and intratumoral vs nontumor, both P = 0.015). TIM-3/CD8 coexpression ...
Immunohistochemistry (IHC) is commonly used for in situ qualitative or quantitative analysis of a target protein in cells of a tissue section. Thought a labeled antibody that is specific to its antigen and sensitive to color-developing agent, this method is also widely used for analyzing the sub...
Polymer-based detection is more sensitive than biotin-based systems. IHC analysis of paraffin-embedded human lung carcinoma using Sox2 (D6D9) XP® Rabbit mAb #3579 and either biotin-based detection (left) or polymer-based detection (SignalStain® Boost IHC Detection Reagent #8114; right). ...
PLK1 (208G4) Rabbit mAb #4513: IHC analysis of paraffin-embedded human colon carcinoma using #4513 with step-by-step reagent substitution, as indicated, demonstrating that reagents can make the difference between poor staining and publishable results. What are the challenges in using IHC? Subopti...
Immunohistochemistry (IHC) is a well-known staining procedure that uses antibodies to detect and localize proteins of interest, such as antigens, within the context of tissue structure. The technique is widely used as an ancillary method in clinical diagnostics to localize and quantify abnormal prote...
IHC analysis of human tonsillitis tissue stained with increasing dilutions of CD68 mouse monoclonal antibody (66231-2-Ig). Dilutions in the range of 1:3200 to 1:6400 was determined to be optimal based on the results. 9. Washing Wash off the primary antibody with wash solution such as PB...