Staphylococcal protein A consists of a single polypeptide with five immunoglobulin G (IgG)-binding domains, which are linked as E-D-A-B-C in this order from the amino terminal. The DNA coding domains A-B were polymerized one to six times linearly, taking advantage of the non-palindromic ...
Staphylococcal protein A consists of a single polypeptide with five immunoglobulin G (IgG)-binding domains, which are linked as E-D-A-B-C in this order from the amino terminal. The DNA coding domains A-B were polymerized one to six times linearly, taking advantage of the non-palindromic nu...
Method for binding immunoglobulin G to protein AA method for binding immunoglobulin G (IgG) to protein A immobilized on an insoluble matrix, wherein a buffer solution having a pH of from 7 to 9.5 containing from 1.0 to 1.6M of a tartarate is used....
Mechanism of formation of the C-terminal β-hairpin of the B3 domain of the immunoglobulin binding protein G from Streptococcus. I. Importance of hydrophob... We previously studied a 16-amino acid-residue fragment of the C-terminal β-hairpin of the B3 domain (residues 46–61), [IG(46–...
Protein A binding + + − + Protein G binding + + + + Reaction with Fc receptors FcγRI + − + + FcγRII + − + − FcγRIII + − + − Attachment to guinea pig skin + − + + (Nezlin, 1994. Reprinted with permission from Marcel Dekker, NY.) Copyrigh...
(Kd) of the protein with respect to human immunoglobulin κ-chain is 400 nM or more at pH 8, or the amino acid sequence of a corresponding immunoglobulin light chain binding domain modified by an amino acid substitution at one or more of the positions equivalent to positions 39, 53 and ...
As the ligand protein to be used for the support, protein A or protein G having the property of binding to the antibody (immunoglobulin) is used. In the production of the antibody drug, the affinity support is used a plurality of times for reduction of the production cost. After using ...
a multicopy plasmid carrying eibG showed chain-like adhesion to HEp-2 cells, and whole-cell lysates of the strain bound to human-derived immunoglobulin G (IgG) Fc and IgA. These results indicate that EibG acts as an IgG Fc- and IgA-binding protein, as well as an adhesin of LEE-...
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Screening and Characterization of Human Immunoglobulin G Binding Peptides Using the ProteOn™ XPR36 Protein Interaction Array System We investigatedthe use of antibody-binding peptides to build a new IgGpurification system by screening a random peptide libraryconstructed in the ... H Takaaki,K Sayak...