avoid the troublesome microscopy step (Yasui and Yoda 1997,Marchet al.2005). Immunological methods still have a lot of unexploited potential (Priest 2003). Various approaches to circumvent the tedious microscopy step have been proposed of which flow cytometry is probably the most widely used. ...
Cell type analysis reveals distinct immune populations associated with five-year survival Following cellular phenotyping by unsupervised clustering, various analyses comparing OS patient cohorts (naïve vs. neoadjuvant chemotherapy-treated specimens, 5-year deceased vs. survival cohorts, etc.) were conducted...
In this study, a cytokine-based combination immunotherapy approach (IRX-2) was evaluated for feasibility, safety, and immunomodulatory activity (including flow cytometry analysis of lymphocyte subsets, T cell repertoire analysis, and assessment of IC infiltration). Treatment included a combination of ...
Amdersom JL,Carlquist JF,Higashiknbo R,et al.Quantitation of lymphocyte subsets by immunofluorescence flow cytometry in idiopathic dilated cardiomyopathy. The American Journal of Cardiology . 1985Quantitation of lymphocyte subsets by immunofluorescence flow cytometry in idiopathic dilated cardiomyopathy. Amd...
This allows users to apply flow cytometry gating strategies to digital microscopy images with access to the original images for visualization. In Fig. 7, we show an example of gating Treg cells in a breast cancer sample using FCS Express. The tissue was stained with IP1, images were acquired...
A quantitative assessment of the expression of estrogen receptors alpha (ERα) is carried out in 115 samples of non-small cell lung cancer (NSCLC) by an immunofluorescent assay and flow cytometry. It is shown that a high level of ERα of ≥20% predicts a higher aggressiveness of NSCLC ...
The immunofluorescence analysis of the tumor-associated protein TUBB3 in gastric cancer tissue and morphologically normal tissue adjacent to the tumor was carried out using flow cytometry (in total 48samples). Expression of TUBB3 was detected in 100% of the tumors and in 79.2% of the normal ...
flow cytometryabsolute countpercentage countAQUIOSThis study is the first that compares the IIF method vs. solid-phase assays in the detection of ANA, on a very large sample size from the routine work-up (real-life approach). Results show that the association of solid-phase CTD screen tests...
immunofluorescence analysisflow cytometryThe expression of PD-L1 protein is quantified in 56 samples of urothelial bladder cancer (BC). PD-L1 expression is detected in all BC samples, while in non-muscle-invasive bladder cancer (NMIBC) this score is 1.5 times higher than in muscle-invasive ...