However, it also highlights some of the disadvantages of gene panels in the rapidly moving field of PID genomics and current challenges in HPO term assignment for PID.doi:10.1111/cge.13163William RaeDaniel WardChristopher MattocksReuben J. Pengelly...
The Next Generation Sequencing workflow was performed to achieve a minimum of 300 × coverage on an Illumina MiSeq (California, United States) platform via a custom designed multi-gene panel (QIAseq Targeted DNA Custom Panels—CDHS-12337Z-606, QIAgen, Hilden, Germany) covering 19 disease-r...
as well as conventional dendritic cells (cDC) and plasmacytoid dendritic cells (pDCs). In addition, we captured hematopoietic stem cells (HSC) and platelets.cViolin plots showing the gene expression levels of selected markers
Many milestones occurred the following year, in 1987: the first antiretroviral drug, zidovudine (AZT), was approved; President Ronald Reagan first discussed HIV/AIDS in a speech to the public; the AIDS quilt went on display, with 1920 panels (Fig. 11.1B and C); and the first HIV vaccine...
51 Among lymphoid malignancies, it has been shown that Ras gene mutations are specifically restricted to multiple myeloma and acute lymphoblastic leukemia.42,52 NHL (not including AIDS-NHL) have been consistently found to lack Ras mutations in panels derived from the United States and Europe42 (...
In Panels B and C, bone marrow and spleen cells were harvested on Day 84 post-transplantation for in vitro expansion and stimulation. Results are from one representative experiment and are expressed as mean ± SEM (n = 3-4). Correction of Defective Immunoglobulin Class Switching in TNF-LT&v...
.fHeatmap representing the expression levels of selected dysregulated genes in CVID activated memory B cells corresponding to the nearest gene to a CVID.no-demeth DMR. Activated naïve B cells and activated memory B cells from CVID-discordant twins are represented. Source data for panels in ...
We employed multiplex real-time PCR to simultaneously detect T-cell receptor excision circles (TRECs), kappa-deleting recombination excision circles (KRECs), and the absence of the survival motor neuron (SMN) 1 gene in dried blood spots from 103,240 newborns in Zhejiang Province, China, between...
Side panels represent individual channels. In C and D, open arrows indicate co-labeled cells; the small arrow in D indicates an infected cell in the lumen of the seminal vesicle epithelium (shown with dotted lines) that did not co-localize with CD3. Scale bars = 50 μm. Download: ...
In contrast, all arterial specimens obtained from HIV-infected individuals with clearly formed atherosclerotic plaque (Figure 1 designated HIVpos/plaque; panels I–L and M–P) contained a population of SMCs, mainly localized in the intima, that were positive for HIV p24 antigen (Figure 1, K ...