PLoS Biol., 19 (2021), Article e3001490, 10.1371/journal.pbio.3001490 View in ScopusGoogle Scholar 66 Y. Zhu, F. Feng, G. Hu, Y. Wang, Y. Yu, Y. Zhu, W. Xu, X. Cai, Z. Sun, W. Han, et al. A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 ...
Antibody binding kinetics were measured by biolayer interferometry (BLI) using a FortéBio Octet HTX instrument (Sartorius). All steps were performed at 25 °C and at an orbital shaking speed of 1000 rpm, and all reagents were formulated in PBSF buffer (1X PBS with 0.1% w/v BSA). All...
Candidate Racrs were chosen on the basis of their similarity of sequence and secondary RNA structure to the relevant CRISPR repeats in the model system (MAFFT alignments, FastTree approximately maximum-likelihood phylogenetic trees; Extended Data Figs.9aand10a–c) and the presence of a promoter sequ...
Bristol Myers Squibb, Celgene, AstraZeneca, Illumina, Genentech, Roche–Ventana, GRAIL, Medicxi, Metabomed, Bicycle Therapeutics and the Sarah Cannon Research Institute; has stock options in ApoGen Biotechnologies, Epic Bioscience and GRAIL; and has stock options in and is a cofounder of Achilles...
Transgenerational immune priming (TGIP), wherein parents transfer immune function to their progeny based upon their own pathogenic experience, has been broadly reported across the animal kingdom and yet, the specific mechanisms underlying TGIP and the evolutionary forces driving TGIP remain poorly unde...
Anti-Mouse PE/Dazzle-conjugated CD11b (clone M1/70) BioLegend Cat#101256; RRID: AB_2563648 Anti-Mouse BV421-conjugated CD3 (clone 17A2) BioLegend Cat#100228; RRID: AB_2562553 Anti-Mouse FITC-conjugated CD3 (clone 17A2) BioLegend Cat#100204; RRID: AB_312661 Anti-Mouse AF700-conjugated...
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& Stock, C. C. The effect of 2,4,6-triethylenimino-s-triazine on the growth of a variety of mouse and rat tumors. Cancer 5, 979–991 (1952). Article CAS PubMed Google Scholar Gouin, K. H. 3rd et al. An N-cadherin 2 expressing epithelial cell subpopulation predicts response to...
Tumour and lymph node lysates were analysed using the Legendplex mouse antivirus response panel (Biolegend) following the manufacturer’s suggested protocol and analysed using the LEGENDplex Data Analysis Software Suite. Four hours after treatment, tumours and the tumour-draining inguinal lymph nodes wer...
PerCP anti-mouse CD45.2 BioLegend Cat# 109826; clone 104; RRID: AB_893349 Pacific Blue anti-mouse CD8a BioLegend Cat# 100725; clone 53–6.7; RRID: AB_493425 Brilliant Violet 605 anti-mouse CD4 BioLegend Cat# 100451; clone GK1.5; RRID:AB_2564591 FITC anti-mouse CD25 BioLegend Cat# 101...