目的评价化合物IMM—H004不同剂量(1.5、3、6mg·k^-1)对大鼠急性全脑缺血/再灌注损伤的保护作用。方法使用四血管结扎法(4VO,20rain)制作大鼠急性全脑缺血/再灌注损伤模型,造模前3天开始尾静脉注射给药(每天1次),参照25分评分法对大鼠进行行为学评分,用尼氏染色方法考察化合物IMM—H004对大鼠海马CAI区、CA3...
化合物IMM-H004对大鼠急性全脑缺血／再灌注损伤的保护作用
目的 利用BiFC双分子荧光互补技术研究香豆素衍生物IMM-H004对趋化素样因子CKLF1与其受体CCR4结合的影响.方法 引入BiFC技术,将CKLF1趋化活性肽段C27与CCR4分别与Venus荧光蛋白的N端(VN173)和C端(VC155)相连,C27与CCR4的结合可以Venus黄色荧光的形式体现,重组载体共转染HEK293细胞,进行氧糖剥夺/复氧损伤造...
基于ＢｉＦＣ技术验证ＩＭＭ Ｈ００４对ＣＫＬＦ１／ＣＣＲ４结合的影响 彭 也１，２，张 钊２，楚世峰２，陈乃宏１ ，２ （１．湖南中医药大学药学院，湖南长沙 ４１０２０８；２．中国医学科学院药物研究所神经科学中心，天然药物活性物质与功能国家重点实验室，北京 １０００５０）收稿日期：２０２０－０３...
A novel coumarin derivative 7-hydroxy-5-methoxy-4-methyl-3-(4-methylpiperazin-1-yl)-coumarin (IMM-H004) has shown anti-apoptotic, anti-inflammatory and neuroprotective activities. In this study we investigated the effects of IMM-H004 on spatial memory in
Furthermore, 1-10 渭M IMM-H004 could decrease the ratio of Bax/Bcl-2 and increase the ratio of p-AKT/AKT, which were related to apoptosis and survival. All these indicated that IMM-H004 protects PC12 cells against H2O2-induced neurotoxicity. Antioxidative and antiapoptosis may be the ...
IMM-H004 citrate (004), 7-hydroxy-5-methoxy-4-methyl-3-(4-methylpiperazin-1-yl)-coumarin, is a coumarin derivative which has potential therapeutic effects in brain ischemia with ambiguous mechanisms. We aim to study the anti-brain ischemia effect and mechanism of 004 in spontaneously ...
IMM-H004Okadaic acidTau proteinNeuroprotectionThis study aimed to explore effects and mechanisms of 004 (IMM-H004), a novel coumarin derivative, in OKA (okadaic acid)-induced AD (Alzheimer's disease)-like model. In vitro, MTT, LDH, and Annexin V/FITC flow cytometry assay were used to ...
Additionally, IMM-H004 significantly suppressed the release of TNF-伪, IL-1尾 and NO, and suppressed the expression of pro-inflammatory mediators and cytokines such as iNOS, COX-2, and IL-6 in LPS-stimulated BV2 microglia. The nuclear translocation of NF-魏B and the phosphorylation level ...
探究香豆素类衍生物IMM-H004对小鼠肝脏缺血再灌注损伤(hepatic ischemia reperfusion injury,HIRI)的保护作用及其机制.本文中所有动物实验都获得中国医学科学院药物研究所伦理学委员会批准.实验分为3组,假手术组,模型组和IMM-H004给药组,检测各组的血清生化指标和病理情况.qPCR检测炎症因子肿瘤坏死因子-α(tumor ...